PXD001571

PXD001571 is an original dataset announced via ProteomeXchange.

Title	Solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) for comprehensive characterization of glycoproteins
Description	A novel chemoenzymatic method termed solid phase extraction of N-linked Glycans And Glycosite-containing peptides (NGAG) for the simultaneous analysis of N-glycans, glycosite-containing peptides, and intact N-glycopeptides with site-specific glycosylation information.
HostingRepository	PRIDE
AnnounceDate	2019-03-25
AnnouncementXML	Submission_2019-03-25_02:51:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paul Aiyetan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-12-04 00:51:52	ID requested
1	2015-11-23 04:04:24	announced
⏵ 2	2019-03-25 02:51:52	announced	Updated publication reference for PubMed record(s): 30884134, 26571101.

Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, H, ö, ti N, Zhang Z, Chan DW, Zhang H, Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotechnol, 34(1):84-8(2016) [pubmed]

Shu J, Dang L, Zhang D, Shah P, Chen L, Zhang H, Sun S, Dynamic analysis of proteomic alterations in response to N-linked glycosylation inhibition in a drug-resistant ovarian carcinoma cell line. FEBS J, 286(8):1594-1605(2019) [pubmed]

ProteomeXchange project tag: CPTAC Consortium

curator keyword: Biomedical

submitter keyword: SPEGAG, SPEG, LC-MSMS, Deglycopeptide, glycosylation, glycosites

Hui Zhang
contact affiliation	Department of Pathology, Johns Hopkins University School of Medicine
contact email	hzhang32@jhmi.edu
lab head
Paul Aiyetan
contact affiliation	Johns Hopkins Medical Institutions
contact email	paiyeta1@jhmi.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD001571

PRIDE project URI

• PRIDE
  1. PXD001571
     1. Label: PRIDE project
     2. Name: Solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) for comprehensive characterization of glycoproteins