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PXD001562

PXD001562 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDYn-2 based identification of Arabidopsis sulfenomes
DescriptionIdentifying the sulfenylation state (-SOH) of stressed cells. Here, we optimized an in vivo trapping method of sulfenic acids in hydrogen peroxide (H2O2) stressed plant cells. With click chemistry, the dimedone based alkyne functionalized DYn-2 probe was biotinylated for subsequent streptavidin enrichment of sulfenylated proteins. the DYn-2 modification and DYn-2-Biotin-azide modification were unknown modifications, we therefore chose N-D-glucuronylglycine [MOD00067] and N-palmitoyl-S-(sn-1-2,3-dipalmitoyl-glycerol)cysteine [MOD00444]
HostingRepositoryPRIDE
AnnounceDate2016-06-27
AnnouncementXMLSubmission_2016-06-27_05:57:35.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD001562
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJarne Pauwels
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListacetylated residue: 42.010565; 2-pyrrolidone-5-carboxylic acid (Gln): -17.026549; monohydroxylated residue: 15.994915; L-cysteic acid (L-cysteine sulfonic acid): 47.984744; N-palmitoyl-S-(sn-1-2: 3-dipalmitoyl-glycerol)cysteine
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-12-02 02:59:55ID requested
12016-06-27 05:57:36announced
Publication List
Akter S, Huang J, Bodra N, De Smet B, Wahni K, Rombaut D, Pauwels J, Gevaert K, Carroll K, Van Breusegem F, Messens J, DYn-2 Based Identification of Arabidopsis Sulfenomes. Mol Cell Proteomics, 14(5):1183-200(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: DYn-2 click chemistry, sulfenome, H2O2, ROS, Arabidopsis, biotinylation
Contact List
Kris Gevaert
contact affiliationVIB Department of Medical Protein Research
contact emailkris.gevaert@vib-ugent.be
lab head
Jarne Pauwels
contact affiliationVIB-Ugent
contact emailjarne.pauwels@vib-ugent.be
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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