PXD001562

PXD001562 is an original dataset announced via ProteomeXchange.

Title	DYn-2 based identification of Arabidopsis sulfenomes
Description	Identifying the sulfenylation state (-SOH) of stressed cells. Here, we optimized an in vivo trapping method of sulfenic acids in hydrogen peroxide (H2O2) stressed plant cells. With click chemistry, the dimedone based alkyne functionalized DYn-2 probe was biotinylated for subsequent streptavidin enrichment of sulfenylated proteins. the DYn-2 modification and DYn-2-Biotin-azide modification were unknown modifications, we therefore chose N-D-glucuronylglycine [MOD00067] and N-palmitoyl-S-(sn-1-2,3-dipalmitoyl-glycerol)cysteine [MOD00444]
HostingRepository	PRIDE
AnnounceDate	2016-06-27
AnnouncementXML	Submission_2016-06-27_05:57:35.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001562
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jarne Pauwels
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue: 42.010565; 2-pyrrolidone-5-carboxylic acid (Gln): -17.026549; monohydroxylated residue: 15.994915; L-cysteic acid (L-cysteine sulfonic acid): 47.984744; N-palmitoyl-S-(sn-1-2: 3-dipalmitoyl-glycerol)cysteine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-12-02 02:59:55	ID requested
⏵ 1	2016-06-27 05:57:36	announced

Akter S, Huang J, Bodra N, De Smet B, Wahni K, Rombaut D, Pauwels J, Gevaert K, Carroll K, Van Breusegem F, Messens J, DYn-2 Based Identification of Arabidopsis Sulfenomes. Mol Cell Proteomics, 14(5):1183-200(2015) [pubmed]

curator keyword: Biological

submitter keyword: DYn-2 click chemistry, sulfenome, H2O2, ROS, Arabidopsis, biotinylation

Kris Gevaert
contact affiliation	VIB Department of Medical Protein Research
contact email	kris.gevaert@vib-ugent.be
lab head
Jarne Pauwels
contact affiliation	VIB-Ugent
contact email	jarne.pauwels@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD001562

PRIDE project URI

• PRIDE
  1. PXD001562
     1. Label: PRIDE project
     2. Name: DYn-2 based identification of Arabidopsis sulfenomes