PXD001562 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | DYn-2 based identification of Arabidopsis sulfenomes |
Description | Identifying the sulfenylation state (-SOH) of stressed cells. Here, we optimized an in vivo trapping method of sulfenic acids in hydrogen peroxide (H2O2) stressed plant cells. With click chemistry, the dimedone based alkyne functionalized DYn-2 probe was biotinylated for subsequent streptavidin enrichment of sulfenylated proteins. the DYn-2 modification and DYn-2-Biotin-azide modification were unknown modifications, we therefore chose N-D-glucuronylglycine [MOD00067] and N-palmitoyl-S-(sn-1-2,3-dipalmitoyl-glycerol)cysteine [MOD00444] |
HostingRepository | PRIDE |
AnnounceDate | 2016-06-27 |
AnnouncementXML | Submission_2016-06-27_05:57:35.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD001562 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Jarne Pauwels |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | acetylated residue: 42.010565; 2-pyrrolidone-5-carboxylic acid (Gln): -17.026549; monohydroxylated residue: 15.994915; L-cysteic acid (L-cysteine sulfonic acid): 47.984744; N-palmitoyl-S-(sn-1-2: 3-dipalmitoyl-glycerol)cysteine |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-12-02 02:59:55 | ID requested | |
⏵ 1 | 2016-06-27 05:57:36 | announced | |
Publication List
Akter S, Huang J, Bodra N, De Smet B, Wahni K, Rombaut D, Pauwels J, Gevaert K, Carroll K, Van Breusegem F, Messens J, DYn-2 Based Identification of Arabidopsis Sulfenomes. Mol Cell Proteomics, 14(5):1183-200(2015) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: DYn-2 click chemistry, sulfenome, H2O2, ROS, Arabidopsis, biotinylation |
Contact List
Kris Gevaert |
contact affiliation | VIB Department of Medical Protein Research |
contact email | kris.gevaert@vib-ugent.be |
lab head | |
Jarne Pauwels |
contact affiliation | VIB-Ugent |
contact email | jarne.pauwels@vib-ugent.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001562
- Label: PRIDE project
- Name: DYn-2 based identification of Arabidopsis sulfenomes