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PXD001560 is an original dataset announced via ProteomeXchange.

Dataset Summary

  • Title: A phosphoproteomic comparison of B-RAFV600E and MKK1/2 inhibitors in melanoma cells, part 1
  • Description: To provide insight into the molecular nature of the clinical responses seen with MAPK pathway inhibition in melanoma, we used quantitative mass spectrometry to characterize the inhibitor-dependent phosphoproteome of human melanoma cells treated with the B-RAFV600E inhibitor PLX4032 (vemurafenib) and the MKK1/2 inhibitor AZD6244 (selumetinib).. In three replicate experiments, we quantified a total of 23,986 phosphosites on 4,722 proteins. This included 1,317 phosphosites that reproducibly decreased in response to at least one inhibitor.
  • HostingRepository: PRIDE
  • AnnounceDate: 2018-01-02
  • AnnouncementXML: Submission_2018-01-02_02:32:50.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original dataset
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Scott Stuart
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
  • Instrument: LTQ Orbitrap Velos

Dataset History

RevisionDatetimeStatusChangeLog Entry
02014-12-02 02:19:44ID requested
12016-02-25 06:15:30announced
22018-01-02 02:32:52announcedUpdated publication reference for PubMed record(s): 25850435, 29255136.

Publication List

  1. Stuart SA, Houel S, Lee T, Wang N, Old WM, Ahn NG, A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics, 14(6):1599-615(2015) [pubmed]
  2. Basken J, Stuart SA, Kavran AJ, Lee T, Ebmeier CC, Old WM, Ahn NG, Specificity of Phosphorylation Responses to Mitogen Activated Protein (MAP) Kinase Pathway Inhibitors in Melanoma Cells. Mol Cell Proteomics, 17(4):550-564(2018) [pubmed]

Keyword List

  1. curator keyword: Biomedical
  2. submitter keyword: Human, Melanoma, SILAC, PLX4032, AZD6244

Contact List

    Natalie G. Ahn
    • contact affiliation: Department of Chemistry and Biochemistry University of Colorado, Boulder Boulder, CO 80309
    • contact email: Natalie.Ahn@colorado.edu
    • lab head:
    Scott Stuart
    • contact affiliation: UC Boulder
    • contact email: scott.stuart@colorado.edu
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List

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