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PXD001560

PXD001560 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA phosphoproteomic comparison of B-RAFV600E and MKK1/2 inhibitors in melanoma cells, part 1
DescriptionTo provide insight into the molecular nature of the clinical responses seen with MAPK pathway inhibition in melanoma, we used quantitative mass spectrometry to characterize the inhibitor-dependent phosphoproteome of human melanoma cells treated with the B-RAFV600E inhibitor PLX4032 (vemurafenib) and the MKK1/2 inhibitor AZD6244 (selumetinib).. In three replicate experiments, we quantified a total of 23,986 phosphosites on 4,722 proteins. This included 1,317 phosphosites that reproducibly decreased in response to at least one inhibitor.
HostingRepositoryPRIDE
AnnounceDate2018-01-02
AnnouncementXMLSubmission_2018-01-02_02:32:50.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterScott Stuart
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-12-02 02:19:44ID requested
12016-02-25 06:15:30announced
22018-01-02 02:32:52announcedUpdated publication reference for PubMed record(s): 25850435, 29255136.
Publication List
Stuart SA, Houel S, Lee T, Wang N, Old WM, Ahn NG, A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics, 14(6):1599-615(2015) [pubmed]
Basken J, Stuart SA, Kavran AJ, Lee T, Ebmeier CC, Old WM, Ahn NG, Specificity of Phosphorylation Responses to Mitogen Activated Protein (MAP) Kinase Pathway Inhibitors in Melanoma Cells. Mol Cell Proteomics, 17(4):550-564(2018) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Human, Melanoma, SILAC, PLX4032, AZD6244
Contact List
Natalie G. Ahn
contact affiliationDepartment of Chemistry and Biochemistry University of Colorado, Boulder Boulder, CO 80309
contact emailNatalie.Ahn@colorado.edu
lab head
Scott Stuart
contact affiliationUC Boulder
contact emailscott.stuart@colorado.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
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