<<< Full experiment listing

PXD001558

PXD001558 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCell-specific analysis of tumour-endothelial contact-intiated signalling
DescriptionTumour cell extravasation is a key event for metastatic colonisation1. However, little is known about the signalling pathways that govern tumour cell adhesion to the endothelium and subsequent transmigration through the blood vessel wall. Here, we combine Stable Isotope Labelling by Amino acids in Cell culture (SILAC) and global phosphoproteomic analysis to identify cell-specific signalling pathways regulated between interacting tumour and endothelial cells. We identified over 2,000 unique phosphorylation sites, where 77 were specifically regulated in the tumour cells and 43 in the endothelial cells.
HostingRepositoryPRIDE
AnnounceDate2016-02-12
AnnouncementXMLSubmission_2016-02-12_03:22:58.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarie Locard-Paulet
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-12-01 07:30:24ID requested
12016-02-12 03:22:59announced
Publication List
Locard-Paulet M, Lim L, Veluscek G, McMahon K, Sinclair J, van Weverwijk A, Worboys JD, Yuan Y, Isacke CM, J, ΓΈ, rgensen C, Phosphoproteomic analysis of interacting tumor and endothelial cells identifies regulatory mechanisms of transendothelial migration. Sci Signal, 9(414):ra15(2016) [pubmed]
Keyword List
curator keyword: Biomedical, Biological
submitter keyword: MDA-MB-231, HUVEC, phospho, contact-initiated signalling, transendothelial migration, Orbitrap Velos, SILAC
Contact List
Claus Jorgensen
contact affiliationSystems Oncology Cancer Research UK Institute, Manchester
contact emailClaus.Jorgensen@cruk.manchester.ac.uk
lab head
Marie Locard-Paulet
contact affiliationCRUK Manchester
contact emailMarie.Locard-Paulet@cruk.manchester.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD001558
PRIDE project URI
Repository Record List
[ + ]