PXD001555

PXD001555 is an original dataset announced via ProteomeXchange.

Title	Differentiation-inducing factor (DIF) SILAC
Description	A global quantitative screen for phosphorylation changes that occur within the first minutes after addition of DIF-1 (differentiation inducing factor) to Dictyostelium cells, using a triple-label SILAC approach. Ax2 cells were labelled in light, medium and heavy medium then starved (5 hr) before DIF treatment. Cells were harvested at 1,5,8,15 minute post treatment to allow time-course of stimulation to be constructed from 2 overlapping triplex experimental samples A(0,1,8 min) amd B(0,5,15) with each experiment repeated in biological triplicate.
HostingRepository	PRIDE
AnnounceDate	2015-01-28
AnnouncementXML	Submission_2015-01-28_08:21:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chris Sugden
SpeciesList	scientific name: Dictyostelium discoideum (Slime mold); NCBI TaxID: 44689;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2014-12-01 02:32:51	ID requested
⏵ 1	2015-01-28 08:21:05	announced

Sugden C, Urbaniak MD, Araki T, Williams JG, The Dictyostelium prestalk inducer differentiation-inducing factor-1 (DIF-1) triggers unexpectedly complex global phosphorylation changes. Mol Biol Cell, 26(4):805-20(2015) [pubmed]

curator keyword: Biological

submitter keyword: dictyostelium, DIF-1, phosphorylation, SILAC

JEFF WILLIAMS
contact affiliation	Cell & Developmental Biology, College of Life Sciences, University of Dundee, UK
contact email	J.G.WILLIAMS@DUNDEE.AC.UK
lab head
Chris Sugden
contact affiliation	University of Dundee
contact email	chrisugden1@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/01/PXD001555

PRIDE project URI

• PRIDE
  1. PXD001555
     1. Label: PRIDE project
     2. Name: Differentiation-inducing factor (DIF) SILAC