PXD001553

PXD001553 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of cathepsin specificity by FPPS
Description	Identification of protease substrates and determination of their specificities can provide important insights into their function. In the past decade, several proteomic approaches for identification of protease cleavage sites were developed, each relying on various strategies for peptide labelling and enrichment. Those approaches often rely on several experimental steps, amongst others involving sample labelling and/or several cycles of chromatographic separation. Here we present a simple and straightforward approach for proteomic identification of protease specificities in a complex proteome background. By combining in solution isotopic labelling with Stage Tip fractionation we developed a simple method to profile protease specificities. We tested our approach by profiling the substrate specificities of the cathepsins K, L and S, three lysosomal proteases known to play important roles in numerous molecular processes.
HostingRepository	PRIDE
AnnounceDate	2015-02-10
AnnouncementXML	Submission_2015-02-10_02:07:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marko Fonovic
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; 3x(2)H residue methyl ester; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-11-28 01:29:42	ID requested
⏵ 1	2015-02-10 02:07:51	announced

Vizovi, š, ek M, Vidmar R, Van Quickelberghe E, Impens F, Andjelkovi, ć U, Soboti, č B, Stoka V, Gevaert K, Turk B, Fonovi, ć M, Fast profiling of protease specificity reveals similar substrate specificities for cathepsins K, L and S. Proteomics, 15(14):2479-90(2015) [pubmed]

curator keyword: Biological

submitter keyword: Degradomics, proteases, cathepsins

Marko Fonovic
contact affiliation	Department of biochemistry, molecular and structural biology Jozef Stefan Institute Ljubljana Slovenia
contact email	marko.fonovic@ijs.si
lab head
Marko Fonovic
contact affiliation	Jozef Stefan Institute
contact email	marko.fonovic@ijs.si
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/02/PXD001553

PRIDE project URI

• PRIDE
  1. PXD001553
     1. Label: PRIDE project
     2. Name: Proteomic profiling of cathepsin specificity by FPPS