PXD001552

PXD001552 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Metarhizium robertsii Lipid Droplets
Description	Metarhizium robertsii is one of the model fungus species widely used studying insect-fungus interactions. Our previous studies have shown the accumulation of lipid droplets (LDs) play an essential role in generation of cellular turgor pressure to assist fungal penetration of insect cuticles, and autophagy-related proteins are connected with LD biogenesis and cellular accumulation in M. robertsii. However, full proteomes of LDs are unclear in terms of the species of proteins involved in lipid biosynthesis and degradation. We performed experiments by growing the fungi in different nutrient conditions, isolated the LDs and extracted the LD proteins from the cultures after being grown in a nutrient-rich medium (i.e. Sabouraud dextrose broth, SDB), a minimum medium without nitrogen source (MM-N) and MM-N supplemented with oleate. The protein samples were then subject to LC-MS/MS proteome profilings for identifying and postulating the proteins/pathways involved in lipid metabolisms.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:48:37.391.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chengshu Wang
SpeciesList	scientific name: Metarhizium robertsii; NCBI TaxID: 568076;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-11-28 01:12:18	ID requested
1	2022-03-01 06:51:46	announced
⏵ 2	2023-11-14 08:48:37	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Metarhizium robertsii, Lipid Droplets, LC-MS/MS

Chengshu Wang
contact affiliation	Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, China
contact email	cswang@sibs.ac.cn
lab head
Chengshu Wang
contact affiliation	Shanghai Institute of Plant Physiology and Ecology, CAS
contact email	cswang@sibs.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD001552

PRIDE project URI

• PRIDE
  1. PXD001552
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Metarhizium robertsii Lipid Droplets