PXD001534

PXD001534 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	IDENTIFICATION OF SUMO-2/3 MODIFIED PROTEINS ASSOCIATED WITH MITOTIC CHROMOSOMES
Description	Sumoylation is essential for progression through mitosis, but the specific protein targets and functions remain poorly understood. In this study, we used chromosome spreads to more precisely define the localization of SUMO-2/3 to the inner-centromere and protein scaffold of mitotic chromosomes. We also developed methods to immune-purify proteins modified by endogenous, untagged SUMO-2/3 from mitotic chromosomes. Using these methods we identified 149 chromosome associated SUMO-2/3 substrates by nLC-ESI-MS/MS. Approximately one-third of the identified proteins have reported functions in mitosis. Consistent with SUMO-2/3 immunolocalization, we identified known centromere and kinetochore associated proteins, as well as chromosome scaffold associated proteins. Notably, >30 proteins involved in chromatin modification or remodeling were identified. Our results provide insights into the roles of sumoylation as a regulator of chromatin structure and other diverse processes in mitosis. Furthermore, our purification and fractionation methodologies represent an important compliment to existing approaches to identify sumoylated proteins using exogenously expressed and tagged SUMOs.
HostingRepository	MassIVE
AnnounceDate	2014-11-24
AnnouncementXML	Submission_2014-11-24_07:33:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tharan Srikumar
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Nethylmaleimide; GG; Oxidation
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2014-11-24 07:32:24	ID requested
⏵ 1	2014-11-24 07:33:03	announced

Publication List

Cube, ñ, as-Potts C, Srikumar T, Lee C, Osula O, Subramonian D, Zhang XD, Cotter RJ, Raught B, Matunis MJ, Identification of SUMO-2/3-modified proteins associated with mitotic chromosomes. Proteomics, 15(4):763-72(2015) [pubmed]

Cube, ñ, as-Potts C, Srikumar T, Lee C, Osula O, Subramonian D, Zhang XD, Cotter RJ, Raught B, Matunis MJ, Identification of SUMO-2/3-modified proteins associated with mitotic chromosomes. Proteomics, 15(4):763-72(2015) [pubmed]

Keyword List

submitter keyword: SUMO, mitosis, chromosome

submitter keyword: SUMO, mitosis, chromosome

Contact List

Michael Matunis
lab head
Tharan Srikumar
contact affiliation	UHN
contact email	tharan.srikumar@gmail.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://MSV000078890:a@massive.ucsd.edu