PXD001526 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Rumen metaproteomics |
Description | Metaproteomic studies of the rumen microbiota are challenged by the need of optimized sample preparation protocols in order to retrieve an enhanced amount of prokaryotic instead of plant and bovine derived cells before protein extraction and subsequent LC-MS/MS analysis. The present study evaluates three different protocols applied to the rumen microbiota either attached to plant fibres or present as planktonic cells. The findings of our work suggest the integration of cheesecloth-gauze filtration in sample preparation to achieve a better protein identification ratio. |
HostingRepository | PRIDE |
AnnounceDate | 2015-04-02 |
AnnouncementXML | Submission_2015-04-02_00:30:29.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jana Seifert |
SpeciesList | scientific name: Eukaryota (eucaryotes); NCBI TaxID: 2759; scientific name: Bacteria; NCBI TaxID: 2; scientific name: Archaea; NCBI TaxID: 2157; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-11-21 02:36:56 | ID requested | |
1 | 2015-04-01 05:46:12 | announced | |
⏵ 2 | 2015-04-02 00:30:31 | announced | Updated project metadata. |
Publication List
Deusch S, Seifert J, Catching the tip of the iceberg - evaluation of sample preparation protocols for metaproteomic studies of the rumen microbiota. Proteomics, 15(20):3590-5(2015) [pubmed] |
Keyword List
curator keyword: Metaproteomics, Technical |
submitter keyword: LC-MS/MS, rumen microbiota, metaproteomics |
Contact List
Jana Seifert |
contact affiliation | University of Hohenheim, Institute of Animal Nutrition |
contact email | jseifert@uni-hohenheim.de |
lab head | |
Jana Seifert |
contact affiliation | Proteomics |
contact email | jseifert@uni-hohenheim.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/04/PXD001526 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001526
- Label: PRIDE project
- Name: Rumen metaproteomics