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PXD001526

PXD001526 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRumen metaproteomics
DescriptionMetaproteomic studies of the rumen microbiota are challenged by the need of optimized sample preparation protocols in order to retrieve an enhanced amount of prokaryotic instead of plant and bovine derived cells before protein extraction and subsequent LC-MS/MS analysis. The present study evaluates three different protocols applied to the rumen microbiota either attached to plant fibres or present as planktonic cells. The findings of our work suggest the integration of cheesecloth-gauze filtration in sample preparation to achieve a better protein identification ratio.
HostingRepositoryPRIDE
AnnounceDate2015-04-02
AnnouncementXMLSubmission_2015-04-02_00:30:29.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJana Seifert
SpeciesList scientific name: Eukaryota (eucaryotes); NCBI TaxID: 2759; scientific name: Bacteria; NCBI TaxID: 2; scientific name: Archaea; NCBI TaxID: 2157;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-11-21 02:36:56ID requested
12015-04-01 05:46:12announced
22015-04-02 00:30:31announcedUpdated project metadata.
Publication List
Deusch S, Seifert J, Catching the tip of the iceberg - evaluation of sample preparation protocols for metaproteomic studies of the rumen microbiota. Proteomics, 15(20):3590-5(2015) [pubmed]
Keyword List
curator keyword: Metaproteomics, Technical
submitter keyword: LC-MS/MS, rumen microbiota, metaproteomics
Contact List
Jana Seifert
contact affiliationUniversity of Hohenheim, Institute of Animal Nutrition
contact emailjseifert@uni-hohenheim.de
lab head
Jana Seifert
contact affiliationProteomics
contact emailjseifert@uni-hohenheim.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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