PXD001525

PXD001525 is an original dataset announced via ProteomeXchange.

Title	Molecular Characterization of LubX. AP-MS using Legionella lysate
Description	Legionella pneumophila delivers over 300 effector proteins into the host cytosol. One of these effectors, LubX contains 2 U-box domains, and directs the proeosomal degradation of another translocated protein, SidH, however this 'metaeffector' activity is pooly understood. As part of our investigation of the interaction of LubX and SidH, we idenified a LubX mutant that was no longer able to abrogate the toxicity of ectopically expressed SidH in yeast. This dataset belongs to AP-MS experiments conducted to test the ability of these mutants for their ability to bind directly to SidH in Legionella lysate mixtures
HostingRepository	PRIDE
AnnounceDate	2020-01-27
AnnouncementXML	Submission_2020-01-27_05:20:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Quaile
SpeciesList	scientific name: Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513); NCBI TaxID: 272624;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2014-11-21 00:45:41	ID requested
⏵ 1	2020-01-27 05:20:52	announced

Quaile AT, Urbanus ML, Stogios PJ, Nocek B, Skarina T, Ensminger AW, Savchenko A, Molecular Characterization of LubX: Functional Divergence of the U-Box Fold by Legionella pneumophila. Structure, 23(8):1459-1469(2015) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Legionella, DITS, LubX, SidH, LTQ XL, AP-MS

Alexei Savchenko
contact affiliation	Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada.
contact email	alexei.savchenko@utoronto.ca
lab head
Andrew Quaile
contact affiliation	University of Toronto
contact email	andy.quaile@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD001525

PRIDE project URI

• PRIDE
  1. PXD001525
     1. Label: PRIDE project
     2. Name: Molecular Characterization of LubX. AP-MS using Legionella lysate