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PXD001524

PXD001524 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman testicular matrix
DescriptionITo derive a scaffold from human testis for the purpose of tissue engineering and regenerative medicine, we developed a method to produce a cytocompatible decellularized testicular matrix (DTM) while maintaining the native tissue-specific characteristics and components. The potential benefits of tissue-specific scaffolds consisting of naturally-derived extracellular matrix (ECM) have been demonstrated using a wide variety of animal and human tissue sources. However, so far, testis scaffolds have never been considered for constructive remodelling purposes. We have therefore developed a protocol for the preparation of cell-free extracellular matrix and characterized the material extensively using a combination of proteomics, immunohistochemsitry, and cell population assays. To prepare cell-free matrix, human cadaveric testicular tissue was exposed for 24 h or 48 h to 1% Triton X-100 and/or 1% sodium dodecyl sulfate (SDS). The extent of decellularization was evaluated by histology. Confirmation of cell removal in DTM was done by a DNA quantification technique. The protein composition was analysed by LC-MS/MS. The retention of testicular tissue-specific characteristics was evaluated by immunohistochemistry, Alcian blue staining and scanning electron microscopy. Soluble toxicity and testicular cell attachment was assessed to check the cytocompatibility of DTM scaffolds. Histological analysis showed that DTM could be obtained by mechanical agitation in 1% SDS for 24 h. The resulting DTM was found to be clear of cells while retaining the typical three-dimensional structure. Proteomics analysis revelaed the presence of the major components of the native tissue scaffold, including collagen type I and IV, fibronectin, laminin and glycosaminoglycans. In addition, numerous additional ECM proteins in DTM were detected, indicating its complex nature. Importantly, we demonstrated that DTM scaffolds are not cytotoxic, as evidenced by MTT assay showing a normal fibroblast proliferation activity after indirect exposure, and support testicular cell attachment and infiltration.
HostingRepositoryPRIDE
AnnounceDate2015-01-30
AnnouncementXMLSubmission_2015-01-30_02:59:16.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD001524
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMichael Landreh
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListOxidation; Carbamidomethyl; Deamidated
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-11-20 07:42:57ID requested
12015-01-30 02:59:18announced
Publication List
Baert Y, Stukenborg JB, Landreh M, De Kock J, J, ö, rnvall H, S, ö, der O, Goossens E, Derivation and characterization of a cytocompatible scaffold from human testis. Hum Reprod, 30(2):256-67(2015) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Extracellular matrix, 3D cell culture, testicular structure
Contact List
Hans Jornvall
contact affiliationMedical Biochemistry and Biophysics, karolinska Institutet, Stockholm, Sweden
contact emailhans.jornvall@ki.se
lab head
Michael Landreh
contact affiliationUniversity of Oxford
contact emailmichael.landreh@chem.ox.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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