PXD001521

PXD001521 is an original dataset announced via ProteomeXchange.

Title	Human “free-N-terminome” analysis
Description	The high throughput characterization of protein N-termini is becoming an emerging challenge in the proteomics and proteogenomics fields. The present study describes the N-terminal oriented analysis of human mitochondria enriched samples by the trimethoxyphenyl phosphonium (TMPP) labeling approach (N-TOP and dN-TOP approaches (Gallien et al, 2009, Bertaccini et al. 2013)). The dN-TOP strategy based on a double light/heavy TMPP labeling has been optimized in order to improve and automate the workflow for efficient, fast and reliable high throughput N-terminome analysis.
HostingRepository	PRIDE
AnnounceDate	2015-06-02
AnnouncementXML	Submission_2015-06-02_07:48:14.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001521
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sebastian VACA
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	amaZon ETD; maXis 4G

Revision	Datetime	Status	ChangeLog Entry
0	2014-11-20 06:28:38	ID requested
⏵ 1	2015-06-02 07:48:15	announced

Vaca Jacome AS, Rabilloud T, Schaeffer-Reiss C, Rompais M, Ayoub D, Lane L, Bairoch A, Van Dorsselaer A, Carapito C, N-terminome analysis of the human mitochondrial proteome. Proteomics, 15(14):2519-24(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Free N-terminome analysis, Human Mitochondria, dN-TOP approach, Proteogenomics

Alain Van Dorsselaer
contact affiliation	Laboratoire de Spectrométrie de Masse BioOrganique (LSMBO), IPHC, Université de Strasbourg, Strasbourg, France
contact email	vandors@unistra.fr
lab head
Sebastian VACA
contact affiliation	CNRS-IPHC-DSA-LSMBO
contact email	sebastian.vaca@etu.unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/06/PXD001521

PRIDE project URI

• PRIDE
  1. PXD001521
     1. Label: PRIDE project
     2. Name: Human “free-N-terminome” analysis