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PXD001509

PXD001509 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEquine synovial fluid .
DescriptionThe aim of the study was to characterise the protein complement of synovial fluid (SF) in health and osteoarthritis (OA) using liquid chromatography mass spectrometry (LC-MS/MS) following peptide-based depletion of high abundance proteins. SF was used from nine normal and nine OA Thoroughbred horses. Samples were analysed with LC-MS/MS using a NanoAcquity™ LC coupled to a LTQ Orbitrap Velos. In order to enrich the lower-abundance protein fractions protein equalisation was first undertaken using ProteoMiner™. Progenesis-QI™ LC-MS software was used for label-free quantification. In addition immunohistochemistry, western blotting and mRNA expression analysis was undertaken on selected joint tissues.The number of protein identifications was increased by 33% in the Proteominer™ treated SF compared to undepleted SF. A total of 754 proteins were identified in SF. A subset of 10 proteins were identified which were differentially expressed in OA SF. S100-A10, a calcium binding protein was upregulated in OA and validated with western blotting and immunohistochemistry. Several new OA specific peptide fragments (neopeptides) were identified.The protein equalisation method compressed the dynamic range of the synovial proteins identifying the most comprehensive SF proteome to date. A number of proteins were identified for the first time in SF which may be involved in the pathogenesis of OA. We identified a distinct set of proteins and neopeptides that may act as potential biomarkers to distinguish between normal and OA joints.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_10:02:01.385.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD001509
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMandy Peffers
SpeciesList scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationListOxidation; Carbamidomethyl
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-11-18 06:59:32ID requested
12024-10-07 10:02:01announced
Publication List
10.6019/PXD001509;
Keyword List
curator keyword: Biological
submitter keyword: osteoarthritis, synovial fluid,equine
Contact List
Robert Beynon
contact affiliationCentre for Proteomic Research, Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool, L69 7ZB
contact emailr.beynon@liverpool.ac.uk
lab head
Mandy Peffers
contact affiliationUniversity of Liverpool
contact emailpeffs@liv.ac.uk
dataset submitter
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Dataset FTP location
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