PXD001507

PXD001507 is an original dataset announced via ProteomeXchange.

Title	Proteogenomic analysis of Anopheles gambiae phagocytic hemocyte populations reveals an anticipatory innate immune response in the absence of pathogen challenge
Description	The innate immune response to a broad class of pathogens is highly conserved across all eukaryotes and has been studied in great detail at the cellular and transcriptomic level in several insect species. However, the commensurate cellular proteomic response, especially of hemocytes, the primary immune cell population in insects, has remained poorly understood. We report on the comprehensive proteogenomic analysis of a phagocyte subpopulation from Anopheles gambiae, the primary malaria mosquito vector in Sub-Saharan Africa. We leveraged the innate phagocytic response of mosquito granulocytes to achieve targeted enrichment for these cells to facilitate the examination of their proteomic response profiles following sugar feeding, as well as non-infectious and infectious blood feeding with Plasmodium falciparum. A comparative integrative-OMICs analysis of existing transcriptomic profiles combined with these proteomic data permitted the delineation of the functional genome of anopheline granulocytes. We observed that phagocytosis, blood feeding, and P. falciparum infection induced dramatic shifts in granulocyte protein expression indicative of broad changes in cellular proliferation and innate immune response priming. Importantly, we identified a large number of hemocyte immune proteins that respond to blood feeding alone, suggesting that granulocytes may play an integral role in an anticipatory immune response prior to immune challenge.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_01:43:19.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001507
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dingyin Tao
SpeciesList	scientific name: Anopheles gambiae (African malaria mosquito); NCBI TaxID: 7165;
ModificationList	Ammonia-loss; Dehydrated; Oxidation; Acetyl; Carbamidomethyl
Instrument	6520 Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2014-11-18 05:41:23	ID requested
⏵ 1	2017-10-24 01:43:20	announced

Smith RC, King JG, Tao D, Zeleznik OA, Brando C, Thallinger GG, Dinglasan RR, Molecular Profiling of Phagocytic Immune Cells in Anopheles gambiae Reveals Integral Roles for Hemocytes in Mosquito Innate Immunity. Mol Cell Proteomics, 15(11):3373-3387(2016) [pubmed]

curator keyword: Biological

submitter keyword: Innate immunity, hemocyte, phagocytosis, mosquito, malaria

Rhoel R. Dinglasan
contact affiliation	W. Harry Feinstone Department of Molecular Microbiology & Immunology, Johns Hopkins Bloomberg School of Public Health
contact email	rdinglasan@jhu.edu
lab head
Dingyin Tao
contact affiliation	W. Harry Feinstone Department of Molecular Microbiology & Immunology
contact email	dtao@jhsph.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001507
     1. Label: PRIDE project
     2. Name: Proteogenomic analysis of Anopheles gambiae phagocytic hemocyte populations reveals an anticipatory innate immune response in the absence of pathogen challenge