PXD001492

PXD001492 is an original dataset announced via ProteomeXchange.

Title	Yeast - phosphoproteome
Description	To find the Mec1 targets that are responsible for mec1-100 suppression on HU, we performed a quantitative phosphoproteomic study. Specifically, we screened for modifications that are downregulated in mec1-100, compensated by pph3delta, and left unaffected by rad53delta. To eliminate contributions from Tel1, we used a tel1delta mec1-100 double mutant in the screen. Prior to extraction of proteins, the cultures were arrested in G1 by alpha-factor and released into S phase in the presence of HU. Samples were analyzed in triplicates.
HostingRepository	PRIDE
AnnounceDate	2015-01-12
AnnouncementXML	Submission_2015-01-12_09:05:25.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001492
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ragna Sack
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	deamidated residue; monohydroxylated residue; iodoacetamide derivatized residue; phosphorylated residue; N-acetylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-11-14 03:45:28	ID requested
⏵ 1	2015-01-12 09:05:26	announced

Hustedt N, Seeber A, Sack R, Tsai-Pflugfelder M, Bhullar B, Vlaming H, van Leeuwen F, Gu, é, nol, é A, van Attikum H, Srivas R, Ideker T, Shimada K, Gasser SM, Yeast PP4 interacts with ATR homolog Ddc2-Mec1 and regulates checkpoint signaling. Mol Cell, 57(2):273-89(2015) [pubmed]

curator keyword: Biological

submitter keyword: S. cereviasiae

Susan M Gasser
contact affiliation	Director of the Friedrich Miescher Institute for Biomedical Research Maulbeerstrasse 66 CH-4058 Basel Switzerland
contact email	susan.gasser@fmi.ch
lab head
Ragna Sack
contact affiliation	Friedrich Miescher Institute for Biomedical Research
contact email	ragna.sack@fmi.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/01/PXD001492

PRIDE project URI

• PRIDE
  1. PXD001492
     1. Label: PRIDE project
     2. Name: Yeast - phosphoproteome