PXD001489

PXD001489 is an original dataset announced via ProteomeXchange.

Title	Protein 2DE reference map of Rhodnius prolixus's anterior midgut, part 2
Description	Rhodnius prolixus is one of the most important vectors of Chagas’ disease caused by the parasite Trypanosoma cruzi. Vector control is the principal combat method of this disease. It is inside of intestine that T. cruzi completes his life cycle to become infectious. On this work a 2D protein map of R. prolixus intestine was performed. On the 2D-map, soluble proteins from crop were separated by two-dimensional gel electrophoresis using 18cm IPG strips pH 4-7 and 12.5% SDS-PAGE gels. After Coomassie blue staining all visible spots were excised, proteins were trypsin digested and analyzed by nano-LC MS/MS. Peptides were identified using in-house Mascot software (Matrixscience) and searched against genomic and transcriptomic databases. 692 spots were picked. Mascot analysis resulted in a total of 476 spots (69%) with a set of 490 proteins identified. These data provide experimental evidence for R. prolixus proteins that were predicted by genome and transcriptome. These proteomic data, together with transcriptomic and genomic information, will contribute in the comprehension of the physiology of R. prolixus midgut.
HostingRepository	PRIDE
AnnounceDate	2015-12-15
AnnouncementXML	Submission_2015-12-14_16:42:59.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001489
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Larissa Rezende Vieira
SpeciesList	scientific name: Rhodnius prolixus (Triatomid bug); NCBI TaxID: 13249;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deaminated residue
Instrument	Q-Tof ultima

Revision	Datetime	Status	ChangeLog Entry
0	2014-11-13 07:08:20	ID requested
⏵ 1	2015-12-14 16:43:00	announced

Vieira LR, Polom, é A, Mesquita RD, Salmon D, Braz GR, Bousbata S, Protein 2DE reference map of the anterior midgut of the blood-sucking bug Rhodnius prolixus. Proteomics, 15(22):3901-4(2015) [pubmed]

curator keyword: Biological

submitter keyword: 2D-map, midgut, Rhodnius prolixus

Sabrina Bousbata
contact affiliation	Proteomic Platform, Laboratory of Molecular Parasitology, Université Libre de Bruxelles, Belgium
contact email	sabrina.bousbata@ulb.ac.be
lab head
Larissa Rezende Vieira
contact affiliation	PhD student
contact email	lrvieira@bioqmed.ufrj.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/12/PXD001489

PRIDE project URI

• PRIDE
  1. PXD001489
     1. Label: PRIDE project
     2. Name: Protein 2DE reference map of Rhodnius prolixus's anterior midgut, part 2