PXD001484

PXD001484 is an original dataset announced via ProteomeXchange.

Title	Douglas fir proteome project
Description	The process of wood formation is of great interest to control and manipulate wood quality for economically important gymnosperms. A Douglas-fir tissue culture system was developed to induce differentiation of callus into tracheary elements (fibers) and to monitor xylogenesisin-vitro by a proteomics approach. Two proteomes were being compared, from an early and late stage during the fiber differentiation process. After 18 weeks in a differentiating medium 80% elongated cells and 20% of cells with advanced spiral thickening were found indicating full wood fiber differentiation. Based on 2D electrophoresis, mass spectrometric, and data analyses, it was shown that nondifferentiated callus (early stage of development) expressed proteins related to protein metabolism, cellular energy and primary cell wall metabolism. At the same time, actively differentiating wood fibers (late stage of development) expressed proteins responsible for housekeeping and stress response, but mostly proteins involved in cell wall polysaccharides biosynthesis.
HostingRepository	PRIDE
AnnounceDate	2015-07-20
AnnouncementXML	Submission_2015-07-20_06:31:51.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001484
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jowita Laniak
SpeciesList	scientific name: Pseudotsuga menziesii var. menziesii; NCBI TaxID: 278161;
ModificationList	iodoacetamide - site C: 57.021464; monohydroxylated residue: 15.994915; deamidated residue: 0.984016
Instrument	Q-Tof ultima

Revision	Datetime	Status	ChangeLog Entry
0	2014-11-12 07:05:42	ID requested
⏵ 1	2015-07-20 06:31:52	announced

Dziedzic JA, McDonald AG, In vitro protein profiles in the early and late stages of Douglas-fir xylogenesis. Electrophoresis, 36(17):2035-45(2015) [pubmed]

curator keyword: Biological

submitter keyword: Dougls fir, proteomics, callus differentiated tracheary elements,

Armando McDoanld
contact affiliation	Department of Forest, Rangeland, and Fire Sciences 875 Perimeter Drive MS 1133 Moscow, Idaho 83844-1133 University of Idaho
contact email	armandm@uidaho.edu
lab head
Jowita Laniak
contact affiliation	Department of Forest, Rangeland, and Fire Sciences
contact email	jlaniak@vandals.uidaho.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/07/PXD001484

PRIDE project URI

• PRIDE
  1. PXD001484
     1. Label: PRIDE project
     2. Name: Douglas fir proteome project