PXD001478

PXD001478 is an original dataset announced via ProteomeXchange.

Title	Proteome analysis of NGF stimulated PC12 cells
Description	We used quantitative mass spectrometry-based proteomics to unravel global nerve growth factor (NGF)-induced changes in protein abundance using the rat PC12 cell line. Cells were stimulated with NGF for 0, 24 and 48 h in a triple SILAC setup (Light: Lys0,Arg0; Medium: Lys4,Arg6; and Heavy: Lys8,Arg10). All experiments were performed as biological replicates.
HostingRepository	PRIDE
AnnounceDate	2015-05-06
AnnouncementXML	Submission_2015-05-06_08:07:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kristina Bennet Emdal
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-11-11 06:26:13	ID requested
⏵ 1	2015-05-06 08:07:14	announced

Emdal KB, Pedersen AK, Bekker-Jensen DB, Tsafou KP, Horn H, Lindner S, Schulte JH, Eggert A, Jensen LJ, Francavilla C, Olsen JV, Temporal proteomics of NGF-TrkA signaling identifies an inhibitory role for the E3 ligase Cbl-b in neuroblastoma cell differentiation. Sci Signal, 8(374):ra40(2015) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Rat, LC-MS/MS, Q Exactive

Jesper Velgaard Olsen
contact affiliation	Novo Nordisk Foundation Center for Protein Research, Faculty of Health & Medical Sciences University of Copenhagen, Blegdamsvej 3B, DK-2200 Copenhagen N, Denmark
contact email	jesper.olsen@cpr.ku.dk
lab head
Kristina Bennet Emdal
contact affiliation	NNF Center for Protein Research, Proteomics Program, Faculty of Health and Medical Sciences, University of Copenhagen.
contact email	kristina.emdal@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/05/PXD001478

PRIDE project URI

• PRIDE
  1. PXD001478
     1. Label: PRIDE project
     2. Name: Proteome analysis of NGF stimulated PC12 cells