PXD001472

PXD001472 is an original dataset announced via ProteomeXchange.

Title	Use of SWATH mass spectrometry for quantitative proteomic investigation of Shewanella oneidensis MR-1 biofilms grown on graphite cloth electrodes
Description	. In this study we show successful use of SWATH-MS for quantitative proteomic analysis of a microbial electrochemically active biofilm. Shewanella oneidensis MR-1 was grown on carbon cloth electrodes under continuous anodic electrochemical polarizations in a bioelectrochemical system. Using lactate as the electron donor, anodes serving as terminal microbial electron acceptors were operated at three different electrode potentials (+0.71V, +0.21V & -0.19V vs. SHE) and the development of catalytic activity was monitored by measuring the current traces over time. Once maximum current was reached (usually within 21-29 hours) the electrochemical systems were shut off and biofilm proteins were extracted from the electrodes for proteomic assessment.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:21:26.105.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christy Grobbler
SpeciesList	scientific name: Shewanella oneidensis (strain MR-1); NCBI TaxID: 211586;
ModificationList	iodoacetamide derivatized amino-terminal residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2014-11-10 02:26:11	ID requested
1	2016-08-10 07:21:09	announced
⏵ 2	2024-10-22 04:21:26	announced	2024-10-22: Updated project metadata.

10.1016/j.syapm.2014.11.007;

Grobbler C, Virdis B, Nouwens A, Harnisch F, Rabaey K, Bond PL, Use of SWATH mass spectrometry for quantitative proteomic investigation of Shewanella oneidensis MR-1 biofilms grown on graphite cloth electrodes. Syst Appl Microbiol, 38(2):135-9(2015) [pubmed]

curator keyword: Technical

submitter keyword: Shewanella oneidensis MR-1, proteomics, bioelectrochemical system

Dr. Philip L. Bond
contact affiliation	The University of Queensland, Advanced Water Management Centre, St. Lucia, QLD 4072, Australia The University of Queensland, School of Chemical and Molecular Biosciences, St. Lucia, QLD 4072, Australia
contact email	phil.bond@awmc.uq.edu.au
lab head
Christy Grobbler
contact affiliation	The University of Queensland
contact email	c.grobbler@uq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD001472

PRIDE project URI

• PRIDE
  1. PXD001472
     1. Label: PRIDE project
     2. Name: Use of SWATH mass spectrometry for quantitative proteomic investigation of Shewanella oneidensis MR-1 biofilms grown on graphite cloth electrodes