PXD001469

PXD001469 is an original dataset announced via ProteomeXchange.

Title	Silkworm cocoon LC-MS/MS
Description	Bombyx mori cocoon has a multi-layer structure that provides optimal protection for silkworm pupa. Research on the mechanical properties of the multi-layer structure revealed structure-property relationships of the cocoon. Here, we investigated the protein components of the B. mori cocoon in terms of its multi-layer structure. Liquid chromatography-tandem mass spectrometry identified 286 proteins from the multiple cocoon layers. In addition to fibroins and sericins, we identified abundant protease inhibitors, seroins and proteins of unknown function. By comparing protein abundance across layers, we found that the outermost layer contained more sericin1 and protease inhibitors and the innermost layer had more seroin1. As many as 36 protease inhibitors were identified in cocoons, indicating efficient inhibitory activities against a fungal protease. Thus, we propose that more abundant protease inhibitors in the outer cocoon layers may provide better protection for the cocoon. This study increases our understanding of the multi-layer mechanism of cocoons, and helps clarify the biological characteristics of cocoons.
HostingRepository	PRIDE
AnnounceDate	2015-04-13
AnnouncementXML	Submission_2015-04-13_01:17:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yan Zhang
SpeciesList	scientific name: Bombyx mori (Silk moth); NCBI TaxID: 7091;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-11-06 05:59:26	ID requested
⏵ 1	2015-04-13 01:17:21	announced

Zhang Y, Zhao P, Dong Z, Wang D, Guo P, Guo X, Song Q, Zhang W, Xia Q, Comparative proteome analysis of multi-layer cocoon of the silkworm, Bombyx mori. PLoS One, 10(4):e0123403(2015) [pubmed]

curator keyword: Biological

submitter keyword: Silkworm, multi-layer cocoon, LC-MS/MS

Qingyou Xia
contact affiliation	State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China
contact email	xiaqy@swu.edu.cn
lab head
Yan Zhang
contact affiliation	State Key Laboratory of Silkworm Genome Biology
contact email	zyid123@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/04/PXD001469

PRIDE project URI

• PRIDE
  1. PXD001469
     1. Label: PRIDE project
     2. Name: Silkworm cocoon LC-MS/MS