PXD001462

PXD001462 is an original dataset announced via ProteomeXchange.

Title	Identification of Alpha-Synuclein Interacting Synaptosomal Proteins
Description	α-Synuclein is an abundant presynaptic protein that when aggregated and dyslocated from the synapse is associated to Parkinson’s disease and dementia. The normal presynaptic function of α-synuclein is unclear as well as the disease triggering mechanism. In order to extend the knowledge of the α-synuclein interactome during normal function and disease, we have used porcine brain synaptosomes as a source of ligands and purified α-synuclein monomers or oligomers as bait in co-immunoprecipitation experiments. The isolated binding synaptosomal proteins were identified with LC-LTQ-orbitrap tandem mass spectrometry and quantified by peak area using the freely-available Windows client application, Skyline Targeted Proteomic Environment. To compare proteins binding to a-synuclein monomer with proteins binding to a-synuclein oligomer, quantifications were log transformed, normalized to buffer-background, and compared by students t-test. Furthermore, to specify the preferential binding an average fold increase was calculated by comparing binding to monomer and oligomer. 10 α-synuclein preferential monomer binding proteins were identified and among those, we successfully validated Abl interactor 1, and myelin proteolipid protein. 76 α-synuclein preferential oligomer binding proteins were found, including glutamate decarboxylase 2, synapsin 1, and glial fibrillary acidic protein, which were positively validated. We identified 92 proteins binding to α-synuclein, for which we were not able to detect any conformational preferences among. This study presents a catalog of proteins interacting with α-synuclein in its non-aggregated and aggregated oligomeric state, which can be used to investigate the normal and pathological roles of α-synuclein.
HostingRepository	PRIDE
AnnounceDate	2015-02-09
AnnouncementXML	Submission_2015-02-09_02:20:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Min Shi
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-11-05 01:26:33	ID requested
⏵ 1	2015-02-09 02:20:48	announced

Betzer C, Movius AJ, Shi M, Gai WP, Zhang J, Jensen PH, -synuclein. PLoS One, 10(2):e0116473(2015) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Alpha-synuclein, Aggregation, Synaptosomes, Protein-protein interactions, Proteomics

Poul Henning Jensen
contact affiliation	University of Aarhus, Denmark
contact email	phj@biokemi.au.dk
lab head
Min Shi
contact affiliation	University of Washington
contact email	mshi70@uw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/02/PXD001462

PRIDE project URI

• PRIDE
  1. PXD001462
     1. Label: PRIDE project
     2. Name: Identification of Alpha-Synuclein Interacting Synaptosomal Proteins