PXD001454

PXD001454 is an original dataset announced via ProteomeXchange.

Title	Investigation of human TFIID subcomplexes 7TAF and 8TAF by cross-linking mass spectrometry
Description	7TAF and 8TAF complexes have been cross-linked with the bifunctional reagent bis(sulfosuccinimidyl)suberate, BS3 that targets mostly lysines . Cross-linked complexes were separated from non-cross-linked species by SDS-PAGE and the product bands were digested in-gel. The cross-linked peptides were analysed by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2015-07-20
AnnouncementXML	Submission_2015-07-20_04:08:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Zou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-10-31 07:02:52	ID requested
⏵ 1	2015-07-20 04:08:49	announced

Trowitzsch S, Viola C, Scheer E, Conic S, Chavant V, Fournier M, Papai G, Ebong IO, Schaffitzel C, Zou J, Haffke M, Rappsilber J, Robinson CV, Schultz P, Tora L, Berger I, Cytoplasmic TAF2-TAF8-TAF10 complex provides evidence for nuclear holo-TFIID assembly from preformed submodules. Nat Commun, 6():6011(2015) [pubmed]

curator keyword: Technical

submitter keyword: 7TAF, 8TAF, Cross-link mass spectrometry (CL-MS)

Juri Rappsilber
contact affiliation	Wellcome Trust Centre for Cell Biology, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JR, United Kingdom. Institute of Bioanalytics, Department of Biotechnology, Technische Universität Berlin, 13353 Berlin, Germany
contact email	juri.rappsilber@ed.ac.uk
lab head
Juan Zou
contact affiliation	The University of Edinburgh
contact email	jzou@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/07/PXD001454

PRIDE project URI

• PRIDE
  1. PXD001454
     1. Label: PRIDE project
     2. Name: Investigation of human TFIID subcomplexes 7TAF and 8TAF by cross-linking mass spectrometry