PXD001452

PXD001452 is an original dataset announced via ProteomeXchange.

Title	The extracellular proteome of two Bifidobacterium strains reveals different adaptation strategies to low iron conditions
Description	B. kashiwanohense PV20-2 and B. pseudolongum PV8-2 are strains isolated from breast fed iron deficient Kenyan infants, selected for their high iron sequestration mechanisms and their genome was completely sequenced. Based on their high iron sequestration features we hypothesized that B. kashiwanohense PV20-2 and B. pseudolongum PV8-2, possess iron related genes and excrete iron binding proteins in the culture media under iron limited conditions. Thus, the complete genomes of B. kashiwanohense PV20-2 and B. pseudolongum PV8-2 were compared to other bifidobacterial genomes to identify genes potentially involved in iron metabolism and the coding sequences from the genome were used as a scaffold to identify the extracellular proteome of both strains grown under low iron conditions using a gel-based shotgun proteomic approach.
HostingRepository	PRIDE
AnnounceDate	2015-12-14
AnnouncementXML	Submission_2015-12-14_08:32:01.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001452
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Pamela Vazquez-Gutierrez
SpeciesList	scientific name: Bifidobacterium pseudolongum; NCBI TaxID: 1694;
ModificationList	monohydroxylated residue; deaminated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-10-31 02:03:53	ID requested
⏵ 1	2015-12-14 08:32:02	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Bifidobacteria, iron, extracellular proteins, B. pseudolongum PV8-2

Prof. Christophe Lacroix
contact affiliation	ETH Zurich
contact email	christophe.lacroix@hest.ethz.ch
lab head
Pamela Vazquez-Gutierrez
contact affiliation	ETH Zurich
contact email	pamela.vazquez@hest.ethz.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001452
     1. Label: PRIDE project
     2. Name: The extracellular proteome of two Bifidobacterium strains reveals different adaptation strategies to low iron conditions