PXD001450

PXD001450 is an original dataset announced via ProteomeXchange.

Title	Characterization and quantification of chicken eggshell proteins during pivotal events of shell calcification
Description	Gallus gallus avian eggshell is composed of 95% calcium carbonate on calcitic form and of 3.5% extracellular organic matrix (proteins, polysaccharides and proteoglycans). This highly ordered structure with a polycrystalline organization result of the control of mineral deposition by the organic matrix components in the lumen of the uterus. This interaction leads to the eggshell ultrastructure and consequently contributes to its resulting mechanical properties. This study used GeLC MS/MS analyses combined to label free quantitative analysis to identify and quantify matrix proteins at the pivotal step of the calcification process (amorphous calcium carbonate deposit, amorphous calcium carbonate transformation into calcite, large calcite crystal units and rapid growth phase). The study gave new insight on proteins playing crucial role in the biomineralization of the shell.
HostingRepository	PRIDE
AnnounceDate	2015-06-09
AnnouncementXML	Submission_2015-06-09_04:15:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	G HARICHAUX
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-10-31 02:00:44	ID requested
⏵ 1	2015-06-09 04:15:54	announced

Marie P, Labas V, Brionne A, Harichaux G, Hennequet-Antier C, Rodriguez-Navarro AB, Nys Y, Gautron J, Quantitative proteomics provides new insights into chicken eggshell matrix protein functions during the primary events of mineralisation and the active calcification phase. J Proteomics, 126():140-54(2015) [pubmed]

curator keyword: Biological

submitter keyword: Chicken, eggshell, organic matrix, LC-MS/MS, proteins, quantification

Joel GAUTRON
contact affiliation	Equipe "Fonction et regulation des proteines de l'oeuf" INRA UR83 Recherches avicoles F-37380 Nouzilly
contact email	joel.gautron@tours.inra.fr
lab head
G HARICHAUX
contact affiliation	INRA-Nouzilly-PRC
contact email	gregoire.harichaux@tours.inra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/06/PXD001450

PRIDE project URI

• PRIDE
  1. PXD001450
     1. Label: PRIDE project
     2. Name: Characterization and quantification of chicken eggshell proteins during pivotal events of shell calcification