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PXD001447

PXD001447 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAP-MS analysis of sirtuin-4 interactions in human fibroblasts
DescriptionSirtuins (SIRTs) form a critical family of nicotinamide adenine dinucleotide (NAD)-dependent enzymes that govern genome regulation, metabolism, and aging. Despite conserved deacetylase domains, SIRTs4-7 have little to no deacetylase activity, and a robust catalytic activity for mitochondrial SIRT4 has remained elusive. Moreover, in vitro characterization of SIRT4 has been hampered by difficulty in maintaining soluble and active recombinant protein. Therefore, to investigate potential cellular substrates of SIRT4, we used proteomics to define its mitochondrial protein interactions in human fibroblasts.
HostingRepositoryPRIDE
AnnounceDate2015-01-06
AnnouncementXMLSubmission_2015-01-06_03:11:42.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD001447
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterT Greco
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListOxidation; Phospho; Dehydrated; Ammonia-loss; Acetyl; Carbamidomethyl; Deamidated
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-10-30 02:19:58ID requested
12015-01-06 03:11:44announced
Publication List
Mathias RA, Greco TM, Oberstein A, Budayeva HG, Chakrabarti R, Rowland EA, Kang Y, Shenk T, Cristea IM, Sirtuin 4 is a lipoamidase regulating pyruvate dehydrogenase complex activity. Cell, 159(7):1615-25(2014) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: SIRT4, sirtuin, protein interactions, PDH
Contact List
Ileana Cristea
contact affiliationPrinceton University, Dept. of Molecular Biology, Princeton, NJ USA
contact emailicristea@princeton.edu
lab head
T Greco
contact affiliationMolecular Biology
contact emailtgreco@princeton.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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