PXD001447 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | AP-MS analysis of sirtuin-4 interactions in human fibroblasts |
Description | Sirtuins (SIRTs) form a critical family of nicotinamide adenine dinucleotide (NAD)-dependent enzymes that govern genome regulation, metabolism, and aging. Despite conserved deacetylase domains, SIRTs4-7 have little to no deacetylase activity, and a robust catalytic activity for mitochondrial SIRT4 has remained elusive. Moreover, in vitro characterization of SIRT4 has been hampered by difficulty in maintaining soluble and active recombinant protein. Therefore, to investigate potential cellular substrates of SIRT4, we used proteomics to define its mitochondrial protein interactions in human fibroblasts. |
HostingRepository | PRIDE |
AnnounceDate | 2015-01-06 |
AnnouncementXML | Submission_2015-01-06_03:11:42.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD001447 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | T Greco |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Oxidation; Phospho; Dehydrated; Ammonia-loss; Acetyl; Carbamidomethyl; Deamidated |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-10-30 02:19:58 | ID requested | |
⏵ 1 | 2015-01-06 03:11:44 | announced | |
Publication List
Mathias RA, Greco TM, Oberstein A, Budayeva HG, Chakrabarti R, Rowland EA, Kang Y, Shenk T, Cristea IM, Sirtuin 4 is a lipoamidase regulating pyruvate dehydrogenase complex activity. Cell, 159(7):1615-25(2014) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: SIRT4, sirtuin, protein interactions, PDH |
Contact List
Ileana Cristea |
contact affiliation | Princeton University, Dept. of Molecular Biology, Princeton, NJ USA |
contact email | icristea@princeton.edu |
lab head | |
T Greco |
contact affiliation | Molecular Biology |
contact email | tgreco@princeton.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/01/PXD001447 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001447
- Label: PRIDE project
- Name: AP-MS analysis of sirtuin-4 interactions in human fibroblasts