PXD001445

PXD001445 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic analyses reveal novel cross-modulation mechanisms between two signaling pathways in yeast
Description	Cells respond to environmental stimuli via specialized signaling pathways. Concurrent stimuli trigger multiple pathways that integrate information, predominantly via protein phosphorylation. Budding yeast respond to NaCl and pheromone via two mitogen-activated protein kinase cascades, the high osmolarity and the mating pathways, respectively. To investigate signal integration between these pathways, we quantified the time-resolved phosphorylation site dynamics after pathway co-stimulation. Using shotgun mass spectrometry, we quantified 2536 phosphopeptides across 36 conditions. Our data indicate that NaCl and pheromone affect phosphorylation events within both pathways, which thus affect each other at more levels than anticipated, allowing for information exchange and signal integration. We observed a pheromone-induced down-regulation of Hog1 phosphorylation due to Gpd1, Ste20, Ptp2, Pbs2 and Ptc1. Distinct Ste20 and Pbs2 phosphosites responded differently to the two stimuli, suggesting these proteins as key mediators of the information exchange. A set of logic models was then used to assess the role of measured phosphopeptides in the crosstalk. Our results show that the integration of the response to different stimuli requires complex interconnections between signaling pathways.
HostingRepository	PRIDE
AnnounceDate	2014-12-19
AnnouncementXML	Submission_2014-12-19_08:34:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefania Vaga
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-10-30 02:08:44	ID requested
⏵ 1	2014-12-19 08:34:28	announced

Vaga S, Bernardo-Faura M, Cokelaer T, Maiolica A, Barnes CA, Gillet LC, Hegemann B, van Drogen F, Sharifian H, Klipp E, Peter M, Saez-Rodriguez J, Aebersold R, Phosphoproteomic analyses reveal novel cross-modulation mechanisms between two signaling pathways in yeast. Mol Syst Biol, 10(12):767(2014) [pubmed]

curator keyword: Biological

submitter keyword: budding yeast, phospho-proteomics, signaling pathways

Ruedi Aebersold
contact affiliation	IMSB, ETH Zurich
contact email	aebersold@imsb.biol.ethz.ch
lab head
Stefania Vaga
contact affiliation	NIMR, MRC
contact email	svaga@nimr.mrc.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/12/PXD001445

PRIDE project URI

• PRIDE
  1. PXD001445
     1. Label: PRIDE project
     2. Name: Phosphoproteomic analyses reveal novel cross-modulation mechanisms between two signaling pathways in yeast