PXD001443

PXD001443 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of dorsal striatum reveals changes in synaptosomal proteins following ethamphetamine self-administration in rats
Description	Methamphetamine is a widely abused, highly addictive drug. Regulation of synaptic proteins within the brain’s reward pathway modulates addiction behaviours, the progression of drug addiction and long-term changes in brain structure and function that result from drug use. Therefore, using large scale proteomics studies we aim to identify global protein expression changes within the dorsal striatum, a key brain region involved in the modulation of addiction. We performed LC-MS/MS analyses on rat striatal synaptosomes following 30 days of methamphetamine self-administration (2 hours/day) and 14 days abstinence. We identified a total of 84 differentially-expressed proteins with known roles in neuroprotection, neuroplasticity, cell cytoskeleton, energy regulation and synaptic vesicles. We identify significant expression changes in stress-induced phosphoprotein and protein Tppp, which have not previously been associated with addiction. In addition, we confirm the role of amphiphysin and phosphatidylethanolamine binding protein in addiction. This approach has provided new insight into the effects of methamphetamine self-administration on synaptic protein expression in a key brain region associated with addiction, showing a large set of differentially-expressed proteins that persist into abstinence.
HostingRepository	PRIDE
AnnounceDate	2015-05-06
AnnouncementXML	Submission_2015-05-06_00:57:25.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001443
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Danyl Mclauchlan
SpeciesList	scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-10-29 02:07:58	ID requested
⏵ 1	2015-05-06 00:57:26	announced

Dataset with its publication pending

curator keyword: Biomedical, Biological

submitter keyword: LC-MS/MS, Rat, Shotgun proteomics, Synaptosome

Lifeng Peng
contact affiliation	School of Biological Sciences Victoria University of Wellington
contact email	lifeng.peng@vuw.ac.nz
lab head
Danyl Mclauchlan
contact affiliation	Centre for Biodiscovery
contact email	danyl.mclauchlan@vuw.ac.nz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/05/PXD001443

PRIDE project URI

• PRIDE
  1. PXD001443
     1. Label: PRIDE project
     2. Name: Proteomics analysis of dorsal striatum reveals changes in synaptosomal proteins following ethamphetamine self-administration in rats