PXD001430

PXD001430 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of urinary exosomes in cystinuria patients
Description	Cystinuria is a rare renal genetic disease caused by mutations in cystine transporter genes and characterized by defective cystine reabsorption leading to kidney stones. In 14% of cases patients undergo nephrectomy, but given the difficulty to predict the evolution of the disease, the identification of markers of kidney damage would improve the follow up of patients with a higher risk. The aim of the present study is to develop a robust, reproducible and non-invasive methodology for proteomic analysis of urinary exosomes using high resolution mass spectrometry. A clinical pilot study, conducted on 8 cystinuria patients vs. 10 controls, highlighted 165 proteins, of which 38 were up-regulated, that separate cystinuria patients from controls, and further discriminate between severe and moderate forms of the disease. These proteins include markers of kidney injury, circulating proteins and a neutrophil signature. Analysis of selected proteins by immunobloting, performed on six additional cystinuria patients, validated the mass spectrometry data. To our knowledge, this is the first successful proteomic study in cystinuria unmasking potential role of inflammation in this disease. The workflow we have developed is applicable to investigate urinanry exosomes in different renal diseases and to search for diagnostic/prognostic markers.
HostingRepository	PRIDE
AnnounceDate	2014-11-17
AnnouncementXML	Submission_2014-11-17_09:37:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiara guerrera
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-10-23 05:05:28	ID requested
⏵ 1	2014-11-17 09:37:24	announced

Bourderioux M, Nguyen-Khoa T, Chhuon C, Jeanson L, Tondelier D, Walczak M, Ollero M, Bekri S, Knebelmann B, Escudier E, Escudier B, Edelman A, Guerrera IC, A new workflow for proteomic analysis of urinary exosomes and assessment in cystinuria patients. J Proteome Res, 14(1):567-77(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Cystinuria, exosome, urine proteomics, Orbitrap

Ida Chiara Guerrera
contact affiliation	Necker Proteomics INSERM US24 University Paris Descartes- Sorbonne Paris Cité
contact email	chiara.guerrera@inserm.fr
lab head
Chiara guerrera
contact affiliation	Faculte de Medecine
contact email	chiara.guerrera@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/11/PXD001430

PRIDE project URI

• PRIDE
  1. PXD001430
     1. Label: PRIDE project
     2. Name: Proteomic analysis of urinary exosomes in cystinuria patients