PXD001404

PXD001404 is an original dataset announced via ProteomeXchange.

Title	Off-line high pH reversed-phase fractionation for in-depth phosphoproteomics
Description	In this study we provide a detailed analysis of the physicochemical characteristics of phosphopeptides, which have been fractionated by off-line high pH chromatography (HpH) before subsequent titanium dioxide (TiO2) enrichment and LC-MS/MS analysis. Our results demonstrate that HpH is superior to standard strong-cation exchange (SCX) fractionation in total number of phosphopeptides detected when analyzing the same number of fractions by identical 70 minutes LC-MS/MS gradients
HostingRepository	PRIDE
AnnounceDate	2014-10-24
AnnouncementXML	Submission_2014-10-24_02:50:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tanveer Batth
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-10-16 02:05:01	ID requested
1	2014-10-23 04:36:50	announced
⏵ 2	2014-10-24 02:50:26	announced	Updated project metadata.

Batth TS, Francavilla C, Olsen JV, Off-line high-pH reversed-phase fractionation for in-depth phosphoproteomics. J Proteome Res, 13(12):6176-86(2014) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Phosphoproteomics

high pH reversed-phase

phosphorylation

peptides

enrichment

fractionation

titanium dioxide

Orbitrap

Jesper V. Olsen
contact affiliation	Novo Nordisk Foundation Center for Protein Research Faculty of Health & Medical Sciences University of Copenhagen Blegdamsvej 3B DK-2200 Copenhagen N Denmark
contact email	jesper.olsen@cpr.ku.dk
lab head
Tanveer Batth
contact affiliation	Novo Nordisk Center for Protein Research, University of Copenhagen
contact email	t.batth@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/10/PXD001404

PRIDE project URI

• PRIDE
  1. PXD001404
     1. Label: PRIDE project
     2. Name: Off-line high pH reversed-phase fractionation for in-depth phosphoproteomics