PXD001394

PXD001394 is an original dataset announced via ProteomeXchange.

Title	Global and specific responses of the histone acetylome to systematic perturbation
Description	Regulation of histone acetylation is fundamental to the utilisation of eukaryotic genomes in chromatin. Aberrant acetylation contributes to disease and can be clinically combated by inhibiting the responsible enzymes. Our knowledge of the histone acetylation system is patchy because we so far lacked the methodology to describe acetylation patterns and their genesis by integrated enzyme activities. We devised a generally applicable, mass spectrometry-based strategy to precisely and accurately quantify combinatorial modification motifs. This was applied to generate a comprehensive inventory of acetylation motifs on histones H3 and H4 in Drosophila cells. Systematic depletion of known or suspected acetyltransferases and deacetylases revealed specific alterations of histone acetylation signatures, established enzyme-substrate relationships and unveiled an extensive crosstalk between neighboring modifications. Unexpectedly, overall histone acetylation levels remained remarkably constant upon depletion of individual acetyltransferases. Conceivably, the acetylation level is adjusted to maintain the global charge neutralisation of chromatin and the stability of nuclei.
HostingRepository	PRIDE
AnnounceDate	2015-01-09
AnnouncementXML	Submission_2015-06-01_06:14:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	N6: N6; acetylated residue; monomethylated residue; dimethylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-10-13 03:07:14	ID requested
1	2015-01-09 07:24:08	announced
⏵ 2	2015-06-01 06:14:27	announced	Updated publication reference for PubMed record(s): 25578876.

Feller C, Forn, é I, Imhof A, Becker PB, Global and specific responses of the histone acetylome to systematic perturbation. Mol Cell, 57(3):559-71(2015) [pubmed]

curator keyword: Biological

submitter keyword: Histone acetylome, LC-MSMS, Drosophila

Prof. Dr. Peter Becker
contact affiliation	Adolf Butenandt Institut Dept of Molecular Biology Ludwig-Maximilians University Schillerstraße 44 80336 Munich (Germany) http://www.molekularbiologie.abi.med.uni-muenchen.de/personen/becker_group/becker/index.html
contact email	pbecker@med.uni-muenchen.de
lab head
Ignasi Forne
contact affiliation	Adolf-Butenandt-Institut
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/01/PXD001394

PRIDE project URI

• PRIDE
  1. PXD001394
     1. Label: PRIDE project
     2. Name: Global and specific responses of the histone acetylome to systematic perturbation