PXD001387 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics of Skin Proteins in Psoriasis |
Description | Herein we demonstrate the efficacy of an unbiased proteomics screening approach for studying protein expression changes in the KC-Tie2 psoriasis mouse model, identifying multiple protein expression changes in the mouse and validating these changes in human psoriasis. KC-Tie2 mouse skin samples (n=3) were compared with littermate controls (n=3) using gel-based fractionation followed by label-free protein expression analysis. 5482 peptides mapping to 1281 proteins were identified and quantitated: 105 proteins exhibited fold-changes ≥2.0 including: stefin A1 (average fold change of 342.4 and an average P = 0.0082; cystatin A, human orthologue); slc25a5 (average fold change of 46.2 and an average P = 0.0318); serpinb3b (average fold change of 35.6 and an average P = 0.0345; serpinB1, human orthologue); and kallikrein related peptidase 6 (average fold change of 4.7 and an average P = 0.2474; KLK6). We independently confirmed mouse gene expression-based increases of selected genes including serpinb3b (17.4-fold, P < 0.0001), KLK6 (9.0-fold, P = 0.002), stefin A1 (7.3-fold; P < 0.001) and slc25A5 (1.5-fold; P = 0.05) using qRT-PCR on a second cohort of animals (n=8). Parallel LC/MS/MS analyses on these same samples verified protein-level increases of 1.3-fold (slc25a5; P < 0.05), 29,000-fold (stefinA1; P < 0.01), 322-fold (KLK6; P < 0.0001) between KC-Tie2 and control mice. To underscore the utility and translatability of our combined approach, we analyzed gene and protein expression levels in psoriasis patient skin and primary keratinocytes vs. healthy controls. Increases in gene expression for slc25a5 (1.8-fold), cystatin A (3.0-fold), KLK6 (5.8-fold) and serpinB1 (76-fold; all P < 0.05) were observed between healthy controls and involved lesional psoriasis skin and primary psoriasis keratinocytes. Moreover slc25a5, cystatin A, KLK6 and serpinB1 protein were all increased in lesional psoriasis skin compared to normal skin. These results highlight the usefulness of preclinical disease models using readily-available mouse skin and demonstrate the utility of proteomic approaches for identifying novel peptides/proteins that are differentially regulated in psoriasis that could serve as sources of auto-antigens or provide novel therapeutic targets for the development of new anti-psoriatic treatments. |
HostingRepository | PRIDE |
AnnounceDate | 2014-10-30 |
AnnouncementXML | Submission_2014-10-30_01:52:50.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD001387 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Kathleen Lundberg |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | Oxidation; Carbamidomethyl |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-10-08 08:26:21 | ID requested | |
⏵ 1 | 2014-10-30 01:52:51 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical, Biological |
submitter keyword: LC-MSMS, Psoriasis, Trangenic Mouse |
Contact List
Mark R. Chance |
contact affiliation | Mark R. Chance, Ph.D. Vice Dean for Research Charles W. and Iona A. Mathias Professor of Cancer Research Director, Center for Proteomics and Bioinformatics School of Medicine Case Western Reserve University 930 BRB, 10900 Euclid Ave. Cleveland OH 44106 |
contact email | mark.chance@case.edu |
lab head | |
Kathleen Lundberg |
contact affiliation | Case Western Reserve University |
contact email | kathleen.lundberg@case.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001387
- Label: PRIDE project
- Name: Proteomics of Skin Proteins in Psoriasis