PXD001383

PXD001383 is an original dataset announced via ProteomeXchange.

Title	Human Transcription Factors LC-MSMS
Description	How transcription factors (TFs), the ultimate targets and executors of cellular signalling pathways, are regulated via protein-protein interactions remains elusive. To systematically investigate the regulations and functions of human transcription factors, we performed proteomics studies of soluble and chromatin-associated complexes of 56 TFs in HEK293T cells, using tandem-affinity-purification followed by mass spectrometry (TAP/MS). We performed 214 purifications and identified 2,156 high-confident protein-protein interactions.
HostingRepository	PRIDE
AnnounceDate	2015-04-08
AnnouncementXML	Submission_2015-04-08_10:21:10.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001383
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Xu Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide - site C: 57.021464; S-carboxamidoethyl-L-cysteine: 71.037114; L-methionine sulfoxide: 15.994915
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2014-10-07 02:59:23	ID requested
⏵ 1	2015-04-08 10:21:11	announced

Li X, Wang W, Wang J, Malovannaya A, Xi Y, Li W, Guerra R, Hawke DH, Qin J, Chen J, Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes. Mol Syst Biol, 11(1):775(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, Transcription Factors, HEK293T, LC-MSMS

Junjie Chen
contact affiliation	Experimental Radiation Oncology, MD Anderson Cancer Center, Houston, TX, USA
contact email	jchen8@mdanderson.org
lab head
Xu Li
contact affiliation	Experimental Radiation Oncology
contact email	xulimda@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/04/PXD001383

PRIDE project URI

• PRIDE
  1. PXD001383
     1. Label: PRIDE project
     2. Name: Human Transcription Factors LC-MSMS