PXD001381 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Highly sensitive phosphoproteomics by tailoring solid-phase extraction to electrostatic repulsion-hydrophilic interaction chromatography |
Description | The dataset contains the data of key experiments for developing a novel and highly sensitive bottom-up phosphoproteomics strategy termed ERLIC-SCX/RP-LC-MS. We compared two SPE types (RP, SCX) for an ERLIC run with 7 fractions. The results were used to design a workflow for highly sensitive bottom-up phosphoproteomics (third experiment) combing ERLIC in 21 fractions with the fractions 1-9 cleaned by SCX-SPE and the fractions 10-21 by RP-SPE. Up to 50% of the fractions were subjected to LC-MS/MS analysis with 45 h of total MS time per replicate. |
HostingRepository | PRIDE |
AnnounceDate | 2014-12-02 |
AnnouncementXML | Submission_2014-12-02_03:21:25.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stefan Loroch |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; iodoacetamide derivatized residue; phosphorylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-10-06 01:18:20 | ID requested | |
⏵ 1 | 2014-12-02 03:21:26 | announced | |
Publication List
Loroch S, Zahedi RP, Sickmann A, Highly sensitive phosphoproteomics by tailoring solid-phase extraction to electrostatic repulsion-hydrophilic interaction chromatography. Anal Chem, 87(3):1596-604(2015) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Protein phosphorylation, Phosphoproteomics, LC-MS, RP, SCX, solid-phase extraction, Phosphopeptide enrichment |
Contact List
Albert Sickmann |
contact affiliation | Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V. Otto-Hahn-Str. 6b, 44227 Dortmund, Germany |
contact email | sickmann@isas.de |
lab head | |
Stefan Loroch |
contact affiliation | Leibniz-Institut für Analytische Wissenschaften, Otto-Hahn-Straße 6b, 44227 Dortmund |
contact email | stefan.loroch@isas.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/12/PXD001381 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001381
- Label: PRIDE project
- Name: Highly sensitive phosphoproteomics by tailoring solid-phase extraction to electrostatic repulsion-hydrophilic interaction chromatography