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PXD001381

PXD001381 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHighly sensitive phosphoproteomics by tailoring solid-phase extraction to electrostatic repulsion-hydrophilic interaction chromatography
DescriptionThe dataset contains the data of key experiments for developing a novel and highly sensitive bottom-up phosphoproteomics strategy termed ERLIC-SCX/RP-LC-MS. We compared two SPE types (RP, SCX) for an ERLIC run with 7 fractions. The results were used to design a workflow for highly sensitive bottom-up phosphoproteomics (third experiment) combing ERLIC in 21 fractions with the fractions 1-9 cleaned by SCX-SPE and the fractions 10-21 by RP-SPE. Up to 50% of the fractions were subjected to LC-MS/MS analysis with 45 h of total MS time per replicate.
HostingRepositoryPRIDE
AnnounceDate2014-12-02
AnnouncementXMLSubmission_2014-12-02_03:21:25.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStefan Loroch
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; iodoacetamide derivatized residue; phosphorylated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-10-06 01:18:20ID requested
12014-12-02 03:21:26announced
Publication List
Loroch S, Zahedi RP, Sickmann A, Highly sensitive phosphoproteomics by tailoring solid-phase extraction to electrostatic repulsion-hydrophilic interaction chromatography. Anal Chem, 87(3):1596-604(2015) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Protein phosphorylation, Phosphoproteomics, LC-MS, RP, SCX, solid-phase extraction, Phosphopeptide enrichment
Contact List
Albert Sickmann
contact affiliationLeibniz-Institut für Analytische Wissenschaften - ISAS - e.V. Otto-Hahn-Str. 6b, 44227 Dortmund, Germany
contact emailsickmann@isas.de
lab head
Stefan Loroch
contact affiliationLeibniz-Institut für Analytische Wissenschaften, Otto-Hahn-Straße 6b, 44227 Dortmund
contact emailstefan.loroch@isas.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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