PXD001375

PXD001375 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of gliosomes from mouse brain for investigation of glial membrane proteins
Description	Astrocytes are increasingly recognized as crucial contributors to neuronal function at synapses, axons, and somas. Reliable methods that can provide insight into the astrocyte proteins at the neuron-astrocyte functional interface are highly desirable. Here we conducted a mass spectrometry analysis of Percoll® gradient isolated gliosomes, a viable preparation of glial subcellular particles often used to study mechanisms of astrocytic transmitter uptake and release and their regulation. Gliosomes were compared with synaptosomes, a preparation containing the neurotransmitter release machinery, and accordingly synaptosomes were enriched for proteins involved in synaptic vesicle mediated transport. Interestingly, gliosome preparations were found to be enriched for different classes of known astrocyte proteins, such as VAMP3 (involved in astrocyte exocytosis), Ezrin (perisynaptic astrocyte cytoskeletal protein), and Basigin (astrocyte membrane glycoprotein), as well as for G-protein mediated signaling proteins. Together, these data provide the first detailed description of the gliosome proteome and show that gliosomes can be a useful preparation to study glial membrane proteins and associated processes.
HostingRepository	PRIDE
AnnounceDate	2014-11-17
AnnouncementXML	Submission_2014-11-17_10:07:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pim van Nierop
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidoethyl-L-cysteine
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2014-10-02 01:56:08	ID requested
⏵ 1	2014-11-17 10:07:29	announced

Carney KE, Milanese M, van Nierop P, Li KW, Oliet SH, Smit AB, Bonanno G, Verheijen MH, Proteomic analysis of gliosomes from mouse brain: identification and investigation of glial membrane proteins. J Proteome Res, 13(12):5918-27(2014) [pubmed]

curator keyword: Biological

submitter keyword: astrocyte proteins, membrane proteins, TripleTOF, gliosome, synaptosome

Dr. Mark H.G. Verheijen
contact affiliation	Dept. Molecular & Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam, VU University Amsterdam, Amsterdam, The Netherlands
contact email	mark.verheijen@vu.nl
lab head
Pim van Nierop
contact affiliation	Molecular and Cellular Neurobiology
contact email	pim.van.nierop@cncr.vu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/11/PXD001375

PRIDE project URI

• PRIDE
  1. PXD001375
     1. Label: PRIDE project
     2. Name: Proteomic analysis of gliosomes from mouse brain for investigation of glial membrane proteins