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PXD001373

PXD001373 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMajor Proteomic Changes Associated with Amyloid Induced Biofilm Formation in Pseudomonas aeruginosa PAO1
DescriptionThe newly identified functional amyloids in Pseudomonas (Fap) are associated with increased aggregation and biofilm formation in the opportunistic pathogen P. aeruginosa. However, whether this phenomenon can be simply ascribed to the mechanical properties of the amyloid fibrils remains undetermined. To gain a deeper understanding of the Fap mediated biofilm formation, the physiological consequences of Fap expression were investigated using label-free protein quantification. The functional amyloids were found to not solely act as inert structural biofilm components. Their presence induced major changes in the global proteome of the bacterium. These included the lowered abundance of classical virulence factors such as elastase B and the secretion system of alkaline protease A. Amyloid mediated biofilm formation furthermore increased abundance of the alginate and pyoverdine synthesis machinery, turning P. aeruginosa PAO1 into an unexpected mucoid phenotype. The results imply a significant impact of functional amyloids in P. aeruginosa for biofilm formation and chronic infections.
HostingRepositoryPRIDE
AnnounceDate2014-10-17
AnnouncementXMLSubmission_2014-10-17_04:18:50.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFlorian-Alexander Herbst
SpeciesList scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-10-01 02:22:29ID requested
12014-10-17 04:18:51announced
Publication List
Herbst FA, S, ø, ndergaard MT, Kjeldal H, Stensballe A, Nielsen PH, Dueholm MS, Major proteomic changes associated with amyloid-induced biofilm formation in Pseudomonas aeruginosa PAO1. J Proteome Res, 14(1):72-81(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Functional amyloids, Pseudomonas aeruginosa PAO1, Microbial proteomics, Biofilms, Cystic fibrosis, Chronic infection
Contact List
Per Halkjær Nielsen
contact affiliationHead of Center for Microbial Communities Department of Biotechnology, Chemistry and Environmental Engineering| Aalborg University | Fredrik Bajersvej 7H | 9220 Aalborg Øst | Denmark
contact emailphn@bio.aau.dk
lab head
Florian-Alexander Herbst
contact affiliationAalborg University
contact emailfah@bio.aau.dk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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