PXD001364 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Widespread Proteome Remodeling and Aggregation in Aging C. elegans |
Description | Aging has been associated with a progressive decline of proteostasis, but how this process affects proteome composition remains largely unexplored. Here, we profiled more than 5,000 proteins along the lifespan of the nematode C. elegans. We find that one-third of proteins change in abundance at least 2-fold during aging, resulting in a severe proteome imbalance. These changes are reduced in the long-lived daf-2mutant but are enhanced in the short-lived daf-16 mutant. While ribosomal proteins decline and lose normal stoichiometry, proteasome complexes increase. Proteome imbalance is accompanied by widespread protein aggregation, with abundant proteins that exceed solubility contributing most to aggregate load. Notably, the properties by which proteins are selected for aggregation differ in the daf-2mutant, and an increased formation of aggregates associated with small heat-shock proteins is observed. We suggest that sequestering proteins into chaperone-enriched aggregates is a protective strategy to slow proteostasis decline during nematode aging. |
HostingRepository | PRIDE |
AnnounceDate | 2015-05-11 |
AnnouncementXML | Submission_2015-05-11_05:04:44.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mario Oroshi |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap; LTQ Orbitrap Velos; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-09-26 01:22:26 | ID requested | |
⏵ 1 | 2015-05-11 05:04:45 | announced | |
Publication List
Walther DM, Kasturi P, Zheng M, Pinkert S, Vecchi G, Ciryam P, Morimoto RI, Dobson CM, Vendruscolo M, Mann M, Hartl FU, Widespread Proteome Remodeling and Aggregation in Aging C. elegans. Cell, 161(4):919-32(2015) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Caenorhabditis elegans, Proteostasis, Aging, Protein Aggregation |
Contact List
Matthias Mann |
contact affiliation | Department of Proteomics and Signal Transduction Max Planck Institute of Biochemistry Germany |
contact email | mmann@biochem.mpg.de |
lab head | |
Mario Oroshi |
contact affiliation | Proteomics |
contact email | oroshi@biochem.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001364
- Label: PRIDE project
- Name: Widespread Proteome Remodeling and Aggregation in Aging C. elegans