PXD001355

PXD001355 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of trypsin-treated extracellular vesicles to evaluate non-vesicular contaminants and surface vesicular proteins.
Description	Extracellular vesicles (EVs) are lipid bilayered vesicles released into extracellular milieu by most of cells. Currently, various purification methods of EVs have been established but most of studies use ultracentrifugation-based purification of EVs. However, these methods have the problem of co-purification with non-vesicular contaminants including various different types of particles, small apoptotic vesicles, protein aggregates, and other derived from dead cells or abundant extracellular proteins. Therefore, evaluation of non-vesicular contaminants gives valuable information in EV research. Here, we purified EVs with high purity using density gradient ultracentrifugation and then purified EVs were treated by trypsin to discriminate extra-vesicular proteins from intra-vesicular proteins.
HostingRepository	PRIDE
AnnounceDate	2021-08-30
AnnouncementXML	Submission_2021-08-30_01:10:50.995.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gyeongyun Go
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	dehydrated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-09-23 01:07:39	ID requested
⏵ 1	2021-08-30 01:10:51	announced

Choi D, Go G, Kim DK, Lee J, Park SM, Di Vizio D, Gho YS, Quantitative proteomic analysis of trypsin-treated extracellular vesicles to identify the real-vesicular proteins. J Extracell Vesicles, 9(1):1757209(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: Quantitative proteomics, extracellular vesicles, non-vesicular contaminants, trypsin treatment

Yong Song Gho
contact affiliation	Department of Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea
contact email	ysgho@postech.ac.kr
lab head
Gyeongyun Go
contact affiliation	POSTECH
contact email	ggy0227@postech.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD001355

PRIDE project URI

• PRIDE
  1. PXD001355
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of trypsin-treated extracellular vesicles to evaluate non-vesicular contaminants and surface vesicular proteins.