PXD001349

PXD001349 is an original dataset announced via ProteomeXchange.

Title	Redox proteomics of Arabidopsis cells under salicylate and flg22 treatments
Description	The redox-sensitive proteins were identified in Arabidopsis cells under the treatment of salicylate or flg22 (a peptide from bacterial flagellin) using OxiTRAQ method. Proteins were extracted from cell samples in the presence of NEM to block free thiols. Reversibly oxidized thiols were reduced by DTT and then tagged with biotin-HPDP. After trypsin digestion and affinity purification of the biotinylated peptides, the different samples were labeled with different iTRAQ reagent and then pooled together. Peptides were identified and quantified by tandem MS. In parallel, total protein abundance was determined by regular iTRAQ assay.
HostingRepository	PRIDE
AnnounceDate	2015-03-03
AnnouncementXML	Submission_2015-03-03_03:17:22.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001349
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Pei Liu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	N-ethylmaleimide derivatized cysteine; monohydroxylated residue; iodoacetamide derivatized residue; iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-09-22 01:27:28	ID requested
⏵ 1	2015-03-03 03:17:23	announced

Liu P, Zhang H, Yu B, Xiong L, Xia Y, Proteomic identification of early salicylate- and flg22-responsive redox-sensitive proteins in Arabidopsis. Sci Rep, 5():8625(2015) [pubmed]

curator keyword: Biological

submitter keyword: Redox protemics, OxiTRAQ, salicylate, flg22

Yiji Xia
contact affiliation	Department of Biology, Hong Kong Baptist University, Hong Kong
contact email	yxia@hkbu.edu.hk
lab head
Pei Liu
contact affiliation	Hong Kong Baptist University
contact email	peiliu0824@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001349
     1. Label: PRIDE project
     2. Name: Redox proteomics of Arabidopsis cells under salicylate and flg22 treatments