PXD001349 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Redox proteomics of Arabidopsis cells under salicylate and flg22 treatments |
Description | The redox-sensitive proteins were identified in Arabidopsis cells under the treatment of salicylate or flg22 (a peptide from bacterial flagellin) using OxiTRAQ method. Proteins were extracted from cell samples in the presence of NEM to block free thiols. Reversibly oxidized thiols were reduced by DTT and then tagged with biotin-HPDP. After trypsin digestion and affinity purification of the biotinylated peptides, the different samples were labeled with different iTRAQ reagent and then pooled together. Peptides were identified and quantified by tandem MS. In parallel, total protein abundance was determined by regular iTRAQ assay. |
HostingRepository | PRIDE |
AnnounceDate | 2015-03-03 |
AnnouncementXML | Submission_2015-03-03_03:17:22.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD001349 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Pei Liu |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | N-ethylmaleimide derivatized cysteine; monohydroxylated residue; iodoacetamide derivatized residue; iTRAQ4plex-116 reporter+balance reagent acylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-09-22 01:27:28 | ID requested | |
⏵ 1 | 2015-03-03 03:17:23 | announced | |
Publication List
Liu P, Zhang H, Yu B, Xiong L, Xia Y, Proteomic identification of early salicylate- and flg22-responsive redox-sensitive proteins in Arabidopsis. Sci Rep, 5():8625(2015) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Redox protemics, OxiTRAQ, salicylate, flg22 |
Contact List
Yiji Xia |
contact affiliation | Department of Biology, Hong Kong Baptist University, Hong Kong |
contact email | yxia@hkbu.edu.hk |
lab head | |
Pei Liu |
contact affiliation | Hong Kong Baptist University |
contact email | peiliu0824@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001349
- Label: PRIDE project
- Name: Redox proteomics of Arabidopsis cells under salicylate and flg22 treatments