PXD001343

PXD001343 is an original dataset announced via ProteomeXchange.

Title	Characterization of the gila monster (Heloderma suspectum suspectum) venom proteome
Description	The archetypical venomous lizard species are the helodermatids, the Gila Monster (Heloderma suspectum) and the Beaded Lizards (Heloderma horridum). In the present study, the gila monster venom proteome was characterized using 2D-gel electrophoresis and tandem mass spectrometry-based de novo peptide sequencing followed by protein identification based on sequence homology. A total of 39 different proteins were identified out of the 58 selected spots that represent the major constituents of venom. Of these proteins, 19 have not previously been identified in helodermatid venom. The data showed that helodermatid venom is complex and that this complexity is caused by genetic isoforms and post-translational modifications including proteolytic processing. In addition, the venom proteome analysis revealed that the major constituents of the gila monster venom are kallikrein-like serine proteinases (EC 3.4.21) and phospholipase A2 (type III) enzymes (EC 3.1.1.4). A neuroendocrine convertase 1 homolog that most likely converts the proforms of the previously identified bioactive exendins into the mature and active forms was identified suggesting that these peptide toxins are secreted as proforms that are activated by proteolytic cleavage following secretion as opposed to being activated intracellularly. The presented global protein identification-analysis provides the first overview of the helodermatid venom composition.
HostingRepository	PRIDE
AnnounceDate	2015-01-31
AnnouncementXML	Submission_2015-01-31_00:31:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas F. Dyrlund
SpeciesList	scientific name: Heloderma suspectum suspectum (Reticulate Gila monster); NCBI TaxID: 709964;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2014-09-18 05:32:55	ID requested
1	2015-01-26 03:01:30	announced
⏵ 2	2015-01-31 00:31:40	announced	Updated project metadata.

Sanggaard KW, Dyrlund TF, Thomsen LR, Nielsen TA, Br, ø, ndum L, Wang T, Th, ø, gersen IB, Enghild JJ, Characterization of the gila monster (Heloderma suspectum suspectum) venom proteome. J Proteomics, 117():1-11(2015) [pubmed]

curator keyword: Biological

submitter keyword: 2D-gel electrophoresis, LC-MS/MS

De novo sequencing, Protein identification, Reptiles, Venomics

Jan Johannes Enghild
contact affiliation	iNANO and Department of Molecular Biology and Genetics, Aarhus University, Denmark
contact email	jje@mb.au.dk
lab head
Thomas F. Dyrlund
contact affiliation	Department of Molecular Biology and Genetics
contact email	tfd@mb.au.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001343
     1. Label: PRIDE project
     2. Name: Characterization of the gila monster (Heloderma suspectum suspectum) venom proteome