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PXD001339

PXD001339 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuh-7 exosomes and HBV
DescriptionHepatitis B virus (HBV) infection could cause hepatitis, liver cirrhosis and hepatocellular carcinoma. HBV-mediated pathogenesis is only partially understood, but X protein (HBx) reportedly possesses oncogenic potential. Exosomes are small membrane vesicles with diverse functions released by various cells including hepatocytes, and HBV harnesses cellular exosome biogenesis and export machineries for virion morphogenesis and secretion. Therefore, HBV infection might cause changes in exosome contents with functional implications for both virus and host. In this project, exosome protein content changes induced by HBV and HBx were quantitatively analyzed by SILAC/LC-MS/MS. Exosomes prepared from SILAC-labeled hepatoma cell line Huh-7 transfected with HBx, wildtype or HBx-null HBV replicon plasmids were analyzed by LC-MS/MS.
HostingRepositoryPRIDE
AnnounceDate2014-10-09
AnnouncementXMLSubmission_2014-10-10_02:04:36.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJing Liu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-09-17 08:22:53ID requested
12014-10-09 08:00:21announced
22014-10-10 02:04:38announcedUpdated publication reference for PubMed record(s): 25265333.
Publication List
Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J, Quantitative proteomic analysis of exosome protein content changes induced by hepatitis B virus in Huh-7 cells using SILAC labeling and LC-MS/MS. J Proteome Res, 13(12):5391-402(2014) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Hepatitis B virus, HBx, exosome
Contact List
Youhua Xie
contact affiliationKey Laboratory of Medical Molecular Virology (MOE/MOH) and Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University
contact emailyhxie@fudan.edu.cn
lab head
Jing Liu
contact affiliationFudan University
contact emailliujing212@fudan.edu.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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