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PXD001335

PXD001335 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComprehensive temporal protein dynamics during the diauxic shift in Saccharomyces cerevisiae
DescriptionYeast (Saccharomyces cerevisea) has served as a key model system in biology and as a benchmark for “omics” technology. Although near-complete proteomes of log phase yeast have been measured, protein abundance in yeast is dynamic, particularly during the transition from log to stationary phase. Defining the dynamics of proteomic changes during this transition, termed the diauxic shift, is important to understand the basic biology of proliferative versus quiescent cells. Here, we perform temporal quantitative proteomics to fully capture protein induction and repression during the diauxic shift. Accurate and sensitive quantitation at a high temporal resolution and depth of proteome coverage was achieved using TMT10 reagents and LC-MS3 analysis on an Orbitrap Fusion tribrid mass spectrometer deploying synchronous precursor selection (SPS). We devised a simple template matching strategy to reveal temporal patterns of protein induction and repression. Within these groups are functionally distinct groups of proteins such as those of glyoxylate metabolism, as well as many proteins of unknown function not previously associated with the diauxic shift (e.g. YNR034W-A and FMP16). We also perform a dual time-course to determine Hap2-dependent proteins during the diauxic shift. These data serve as an important basic model for fermentative versus respiratory growth of yeast and other eukaryotes and are a benchmark for temporal quantitative proteomics.
HostingRepositoryPRIDE
AnnounceDate2015-06-20
AnnouncementXMLSubmission_2015-06-19_17:43:41.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPatrick Murphy
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListiodoacetamide derivatized residue; TMT6plex-126 reporter+balance reagent acylated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-09-17 02:01:51ID requested
12015-06-19 17:43:42announced
Publication List
Murphy JP, Stepanova E, Everley RA, Paulo JA, Gygi SP, Comprehensive Temporal Protein Dynamics during the Diauxic Shift in Saccharomyces cerevisiae. Mol Cell Proteomics, 14(9):2454-65(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Yeast, Temporal Data, TMT
Contact List
Steve Gygi
contact affiliationHarvard Medical School
contact emailsteven_gygi@hms.harvard.edu
lab head
Patrick Murphy
contact affiliationMass Spectrometry
contact emailpitter9@gmail.com
dataset submitter
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Dataset FTP location
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