PXD001330

PXD001330 is an original dataset announced via ProteomeXchange.

Title	Temporal quantitative proteomics of Chikungunya virus infected cells
Description	To better understand the complex CHIKV-host interplay we used stable isotope labeling with amino acids in cell culture (SILAC) and LC-MS/MS to define the temporal dynamics of the cellular response to infection. Using samples harvested at 8, 10 and 12 hours post infection, over 4700 proteins were identified and per time point 2750-3150 proteins could be quantified in both biological replicates.
HostingRepository	PRIDE
AnnounceDate	2015-03-16
AnnouncementXML	Submission_2015-04-15_02:09:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emmely Treffers
SpeciesList	scientific name: Cercopithecidae (Old World monkeys); NCBI TaxID: 9527;
ModificationList	monohydroxylated residue; 6x(13)C: 4x(15)N labeled L-arginine; acetylated residue; iodoacetamide derivatized residue; 6x(13)C: 2x(15)N labeled L-lysine
Instrument	LTQ FT Ultra

Revision	Datetime	Status	ChangeLog Entry
0	2014-09-16 05:59:18	ID requested
1	2015-03-16 05:02:36	announced
⏵ 2	2015-04-15 02:09:44	announced	Updated publication reference for PubMed record(s): 25764339.

Treffers EE, Tas A, Scholte FE, Van MN, Heemskerk MT, de Ru AH, Snijder EJ, van Hemert MJ, van Veelen PA, Temporal SILAC-based quantitative proteomics identifies host factors involved in chikungunya virus replication. Proteomics, 15(13):2267-80(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: LC-MSMS, SILAC, quantitative proteomics, Chikungunya virus

Peter A van Veelen
contact affiliation	Department of Immunohematology and Blood Transfusion, Leiden University Medical Center
contact email	p.a.van_veelen@lumc.nl
lab head
Emmely Treffers
contact affiliation	LUMC
contact email	e.e.treffers@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/03/PXD001330

PRIDE project URI

• PRIDE
  1. PXD001330
     1. Label: PRIDE project
     2. Name: Temporal quantitative proteomics of Chikungunya virus infected cells