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PXD001327

PXD001327 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome profiling of breast cancer biopsies reveals a wound healing signature of cancer-associated fibroblasts
DescriptionBreast cancer is still the most common type of cancer in women; an important role in carcinogenesis is actually attributed to cancer-associated fibroblasts. In this study we investigated whether it is possible to assess the functional state of cancer-associated fibroblasts through tumor tissue proteome profiling. Tissue proteomics was performed on tumor-central, tumor-near and tumor-distant biopsy sections from breast adenocarcinoma patients, which allowed us to identify 2074 proteins. Data were interpreted referring to reference proteome profiles generated from primary human mammary fibroblasts comprising 4095 proteins. These cells were analyzed in quiescent cell state, as well as after in vitro treatment with TGFβ or IL-1β, stimulating wound healing or inflammatory processes, respectively. Representative for cancer cells, we investigated the mammary carcinoma cell line ZR-75-1, identifying 5212 proteins. Comparison of tissue proteomics data with all those reference profiles revealed predominance of cancer cell-derived proteins within the tumor and fibroblast-derived proteins in the tumor-distant tissue sections. Remarkably, proteins characteristic for acute inflammation were hardly identified in the tissue samples. In contrast, several proteins found by us to be induced by TGFβ in mammary fibroblasts, including fibulin-5, SLC2A1 and MUC18, were positively identified in all tissue samples, with relatively higher abundance in tumor neighboring tissue sections. These findings indicate a predominance of cancer-associated fibroblasts with wound healing activities localized around tumors.
HostingRepositoryPRIDE
AnnounceDate2014-09-23
AnnouncementXMLSubmission_2014-09-23_06:15:38.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD001327
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChristopher Gerner
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListOxidation; Acetyl; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-09-16 05:10:56ID requested
12014-09-23 06:15:39announced
Publication List
Groessl M, Slany A, Bileck A, Gloessmann K, Kreutz D, Jaeger W, Pfeiler G, Gerner C, Proteome profiling of breast cancer biopsies reveals a wound healing signature of cancer-associated fibroblasts. J Proteome Res, 13(11):4773-82(2014) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Breast cancer, cancer-associated fibroblasts, comparative proteome profiling, label-free quantification, primary human cells, tissue proteomics.
Contact List
Christopher Gerner
contact affiliationUniversity of Vienna, Faculty of Chemistry, Department of Analytical Chemistry
contact emailchristopher.gerner@univie.ac.at
lab head
Christopher Gerner
contact affiliationUniversity of Vienna
contact emailchristopher.gerner@univie.ac.at
dataset submitter
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Dataset FTP location
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