PXD001305

PXD001305 is an original dataset announced via ProteomeXchange.

Title	Rapid and Deep Proteomes by Faster Sequencing on a Benchtop Quadrupole Ultra-High-Field Orbitrap Mass Spectrometer
Description	The faster sequencing speed available on the latest Q Exactive HF mass spectrometer is investigated and evaluated by four different acquisition methods and benchmarked across three generations of Q Exactive instruments. Also the instrument capabilities for offline high pH reversed-phase peptide fractionation and single-shot phosphoproteomics are evaluated.
HostingRepository	PRIDE
AnnounceDate	2014-10-31
AnnouncementXML	Submission_2014-10-31_04:42:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Kelstrup
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-09-11 06:55:11	ID requested
⏵ 1	2014-10-31 04:42:21	announced

Kelstrup CD, Jersie-Christensen RR, Batth TS, Arrey TN, Kuehn A, Kellmann M, Olsen JV, Rapid and deep proteomes by faster sequencing on a benchtop quadrupole ultra-high-field Orbitrap mass spectrometer. J Proteome Res, 13(12):6187-95(2014) [pubmed]

ProteomeXchange project tag: PRIME-XS Project

curator keyword: Biomedical

submitter keyword: Orbitrap. Q Exactive HF. Shotgun proteomics. HCD. HeLa. Phosphoproteomics. Single-shot analysis. High pH Reversed-phase fractionation. Parallel acquisition. Deep proteome coverage

Jesper V. Olsen
contact affiliation	NNF Center for Protein Research, University of Copenhagen
contact email	jesper.olsen@cpr.ku.dk
lab head
Christian Kelstrup
contact affiliation	University of Copenhagen
contact email	chdake@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/10/PXD001305

PRIDE project URI

• PRIDE
  1. PXD001305
     1. Label: PRIDE project
     2. Name: Rapid and Deep Proteomes by Faster Sequencing on a Benchtop Quadrupole Ultra-High-Field Orbitrap Mass Spectrometer