PXD001304

PXD001304 is an original dataset announced via ProteomeXchange.

Title	Trichoderma RUT C-30 secretome after growth on different cellulosic substrates
Description	Enzyme production by T. reesei Rut C-30 was studied in submerged cultures on five different cellulose-rich substrates, namely, commercial cellulose Avicel® and industrial-like cellulosic pulp substrates which consist mainly of cellulose, but also contain residual hemicellulose and lignin. In order to evaluate the hydrolysis of the substrates by the fungal enzymes, the spatial polymer distributions were characterised by cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C-NMR) in combination with spectral fitting. Proteins in culture supernatants at early and late stages of enzyme production were labeled by Tandem Mass Tags (TMT) and protein profiles were analysed by liquid chromatography-tandem mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2014-09-11
AnnouncementXML	Submission_2014-09-11_04:07:33.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001304
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	George Anasontzis
SpeciesList	scientific name: Trichoderma reesei RUT C-30; NCBI TaxID: 1344414;
ModificationList	monohydroxylated residue; methylthiolated residue; TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-09-10 08:06:59	ID requested
⏵ 1	2014-09-11 04:07:34	announced

Peciulyte A, Anasontzis GE, Karlstr, ö, m K, Larsson PT, Olsson L, Morphology and enzyme production of Trichoderma reesei Rut C-30 are affected by the physical and structural characteristics of cellulosic substrates. Fungal Genet Biol, 72():64-72(2014) [pubmed]

curator keyword: Biological

submitter keyword: cellulose I structure, submerged cultivation, biomass degrading enzymes, Tandem Mass Tags, quantitative proteomics

Lisbeth Olsson
contact affiliation	Industrial Biotechnology, Chalmers University of Technology
contact email	lisbeth.olsson@chalmers.se
lab head
George Anasontzis
contact affiliation	Chalmers University of Technology
contact email	george.anasontzis@chalmers.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001304
     1. Label: PRIDE project
     2. Name: Trichoderma RUT C-30 secretome after growth on different cellulosic substrates