PXD001293

PXD001293 is an original dataset announced via ProteomeXchange.

Title	Comaprison of the phagosome proteome of bone marrow-derived macrophages and RAW264.7
Description	Macrophages are important immune cells operating at the forefront of innate immunity by taking up foreign particles and microbes through phagocytosis. The RAW 264.7 cell line is commonly used for experiments in the macrophage and phagocytosis field. However, little is known how its functions compare to primary macrophages. Here, we have performed an in-depth proteomics characterisation of phagosomes from RAW 264.7 and bone marrow-derived macrophages by quantifying more than 2500 phagosomal proteins. Our data indicates that there are significant differences for a large number of proteins including important receptors such as mannose receptor 1 and Siglec-1.
HostingRepository	PRIDE
AnnounceDate	2015-02-10
AnnouncementXML	Submission_2015-02-10_02:19:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthias Trost
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetic acid derivatized residue; monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-09-08 08:17:17	ID requested
⏵ 1	2015-02-10 02:19:02	announced

Guo M, H, ä, rtlova A, Dill BD, Prescott AR, Gierli, ń, ski M, Trost M, High-resolution quantitative proteome analysis reveals substantial differences between phagosomes of RAW 264.7 and bone marrow derived macrophages. Proteomics, 15(18):3169-74(2015) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: macrophage, phagosome, mouse, RAW264.7

Matthias Trost
contact affiliation	MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, DD1 5EH, Scotland, United Kingdom.
contact email	m.trost@dundee.ac.uk
lab head
Matthias Trost
contact affiliation	MRC Protein Phosphorylation Unit
contact email	m.trost@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001293
     1. Label: PRIDE project
     2. Name: Comaprison of the phagosome proteome of bone marrow-derived macrophages and RAW264.7