PXD001292 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comprehensive phosphoproteome analysis unravels the core signaling network that initiates the earliest synapse pathology in preclinical Alzheimer's disease brain |
Description | Using a high-end mass spectrometry, we screened phosphoproteins and phosphopeptides in four types of Alzheimer's disease (AD) mouse models and human AD postmortem brains. We identified commonly changed phosphoproteins in multiple models and also determined phosphoproteins related to initiation of Abeta deposition in the mouse brain. We put the proteins on experimentally verified protein-protein interaction databases. Surprisingly most of the core phosphoproteins were directly connected, and they formed a functional network linked to synaptic spine formation. The change of the core network started at a preclinical stage even before histological Abeta deposition. Systems biology analyses suggested phosphorylation of MARCKS by over-activated kinases including PKCs and CaMKs initiates synapse pathology. |
HostingRepository | PRIDE |
AnnounceDate | 2014-09-10 |
AnnouncementXML | Submission_2014-09-22_06:01:23.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hidenori Homma |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iTRAQ8plex-116 reporter+balance reagent acylated residue; phosphorylated residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-09-08 02:20:22 | ID requested | |
1 | 2014-09-10 04:13:58 | announced | |
⏵ 2 | 2014-09-22 06:01:24 | announced | Updated publication reference for PubMed record(s): 25231903. |
Publication List
Tagawa K, Homma H, Saito A, Fujita K, Chen X, Imoto S, Oka T, Ito H, Motoki K, Yoshida C, Hatsuta H, Murayama S, Iwatsubo T, Miyano S, Okazawa H, Comprehensive phosphoproteome analysis unravels the core signaling network that initiates the earliest synapse pathology in preclinical Alzheimer's disease brain. Hum Mol Genet, 24(2):540-58(2015) [pubmed] |
Keyword List
curator keyword: Biomedical, Biological |
submitter keyword: phosphoproteome, preclinical Alzheimer's disease, signaling network, kinases, PKCs, CaMKs |
Contact List
Hitoshi Okazawa |
contact affiliation | Department of Neuropathology, Medical Research institute and Center for Brain Integration Research, Tokyo Medical and Dental University |
contact email | okazawa-tky@umin.ac.jp |
lab head | |
Hidenori Homma |
contact affiliation | Tokyo Medical and Dental University |
contact email | homma.npat@mri.tmd.ac.jp |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001292
- Label: PRIDE project
- Name: Comprehensive phosphoproteome analysis unravels the core signaling network that initiates the earliest synapse pathology in preclinical Alzheimer's disease brain