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PXD001292

PXD001292 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComprehensive phosphoproteome analysis unravels the core signaling network that initiates the earliest synapse pathology in preclinical Alzheimer's disease brain
DescriptionUsing a high-end mass spectrometry, we screened phosphoproteins and phosphopeptides in four types of Alzheimer's disease (AD) mouse models and human AD postmortem brains. We identified commonly changed phosphoproteins in multiple models and also determined phosphoproteins related to initiation of Abeta deposition in the mouse brain. We put the proteins on experimentally verified protein-protein interaction databases. Surprisingly most of the core phosphoproteins were directly connected, and they formed a functional network linked to synaptic spine formation. The change of the core network started at a preclinical stage even before histological Abeta deposition. Systems biology analyses suggested phosphorylation of MARCKS by over-activated kinases including PKCs and CaMKs initiates synapse pathology.
HostingRepositoryPRIDE
AnnounceDate2014-09-10
AnnouncementXMLSubmission_2014-09-22_06:01:23.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHidenori Homma
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiTRAQ8plex-116 reporter+balance reagent acylated residue; phosphorylated residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-09-08 02:20:22ID requested
12014-09-10 04:13:58announced
22014-09-22 06:01:24announcedUpdated publication reference for PubMed record(s): 25231903.
Publication List
Tagawa K, Homma H, Saito A, Fujita K, Chen X, Imoto S, Oka T, Ito H, Motoki K, Yoshida C, Hatsuta H, Murayama S, Iwatsubo T, Miyano S, Okazawa H, Comprehensive phosphoproteome analysis unravels the core signaling network that initiates the earliest synapse pathology in preclinical Alzheimer's disease brain. Hum Mol Genet, 24(2):540-58(2015) [pubmed]
Keyword List
curator keyword: Biomedical, Biological
submitter keyword: phosphoproteome, preclinical Alzheimer's disease, signaling network, kinases, PKCs, CaMKs
Contact List
Hitoshi Okazawa
contact affiliationDepartment of Neuropathology, Medical Research institute and Center for Brain Integration Research, Tokyo Medical and Dental University
contact emailokazawa-tky@umin.ac.jp
lab head
Hidenori Homma
contact affiliationTokyo Medical and Dental University
contact emailhomma.npat@mri.tmd.ac.jp
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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