PXD001286

PXD001286 is an original dataset announced via ProteomeXchange.

Title	The dynamics of protein-bound methionine oxidation events in Arabidopsis thaliana during oxidative stress
Description	Reactive oxygen species such as hydrogen peroxide can modify proteins via direct oxidation of their sulfur-containing amino acids, cysteine and methionine. Methionine oxidation, studied here, is a reversible posttranslational modification, which is increasingly suggested as a mechanism by which proteins perceive oxidative stress and function in redox signalling. Identification of proteins with oxidized methionines is a first prerequisite towards understanding the functional effect of methionine oxidation on proteins and the biological processes in which they are involved. Here, we describe a proteome-wide study of in vivo protein-bound methionine oxidation in plants upon oxidative stress, using Arabidopsis thaliana catalase 2 knock-out plants as a model system. We identified approximately 500 sites of oxidation in about 400 proteins, and quantified the differences in oxidation between wild-type and catalase 2 knock-out plants. Further, by sampling over time, we mapped the dynamics of methionine oxidation and gained new insights into this complex and dynamic landscape of the part of the plant proteome that is sculpted by oxidative stress.
HostingRepository	PRIDE
AnnounceDate	2015-03-24
AnnouncementXML	Submission_2015-03-24_07:33:46.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001286
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jonathan Vandenbussche
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	S-carboxymethyl-L-cysteine: 58.005479; propanoyl labeling reagent heavy form (+3amu) (K): 59.036279; 2-pyrrolidone-5-carboxylic acid (Gln): -17.026549; N-acetylated residue: 42.010565; L-methionine (S)-sulfoxide: 15.994915; propanoyl labeling reagent light form (N-term): 56.026215; N6-propanoyl-L-lysine: 56.026215
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-09-04 02:46:44	ID requested
⏵ 1	2015-03-24 07:33:47	announced

Jacques S, Ghesqui, è, re B, De Bock PJ, Demol H, Wahni K, Willems P, Messens J, Van Breusegem F, Gevaert K, Protein Methionine Sulfoxide Dynamics in Arabidopsis thaliana under Oxidative Stress. Mol Cell Proteomics, 14(5):1217-29(2015) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis Thaliana, oxidative stress, methionine oxidation, catalase mutant

Kris Gevaert
contact affiliation	VIB, department of Medical Protein Research Ugent, department of Biochemistry
contact email	kris.gevaert@vib-ugent.be
lab head
Jonathan Vandenbussche
contact affiliation	Biochemistry
contact email	jonathan.vandenbussche@vib-ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001286
     1. Label: PRIDE project
     2. Name: The dynamics of protein-bound methionine oxidation events in Arabidopsis thaliana during oxidative stress