PXD001284

PXD001284 is an original dataset announced via ProteomeXchange.

Title	The Quantitative Changes in the Yeast Hsp70 and Hsp90 Interactomes upon DNA Damage
Description	The molecular chaperones Hsp70 and Hsp90 participate in many important cellular processes, including how cells respond to DNA damage. In this study, we applied quantitative affinity-purification mass spectrometry (AP-MS) proteomics to understand the protein network through which Hsp70 and Hsp90 exert their effects on the DNA damage response (DDR). We characterized the interactomes of the yeast Hsp70 isoform Ssa1 and Hsp90 isoform Hsp82 before and after exposure to methyl methanesulfonate. We identified 256 chaperone interactors, 146 of which are novel. Although the majority of chaperone interaction remained constant under DNA damage, 5 proteins (Coq5, Ast1, Cys3, Ydr210c and Rnr4) increased in interaction with Ssa1 and/or Hsp82. This data is related to article “Quantitative proteomics of the yeast Hsp70/Hsp90 interactomes during DNA damage reveals chaperone-dependent regulation of ribonucleotide reductase”.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:04:14.971.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Donald Wolfgeher
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	L-methionine sulfone; (18)O label at both C-terminal oxygens; formylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-09-04 01:57:50	ID requested
1	2014-12-08 08:00:15	announced
⏵ 2	2024-10-22 04:04:21	announced	2024-10-22: Updated project metadata.

Truman AW, Kristjansdottir K, Wolfgeher D, Ricco N, Mayampurath A, Volchenboum SL, Clotet J, Kron SJ, Quantitative proteomics of the yeast Hsp70/Hsp90 interactomes during DNA damage reveal chaperone-dependent regulation of ribonucleotide reductase. J Proteomics, 112():285-300(2015) [pubmed]

10.1016/j.jprot.2014.09.028;

curator keyword: Biological

submitter keyword: Saccharomyces cerevisiae,18O Quantitation, LTQ Orbitrap XL, LC-MS/MS

Stephen J. Kron
contact affiliation	Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA
contact email	skron@uchicago.edu
lab head
Donald Wolfgeher
contact affiliation	University of Chicago
contact email	donw@uchicago.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001284
     1. Label: PRIDE project
     2. Name: The Quantitative Changes in the Yeast Hsp70 and Hsp90 Interactomes upon DNA Damage