PXD001277

PXD001277 is an original dataset announced via ProteomeXchange.

Title	Mouse, Hippocampus, LC-MSMS
Description	Omega-3 fatty acid (n-3 FA) deficiency is an environmental risk factor for schizophrenia, yet characterisation of the consequences of deficiency at the protein level in the brain is limited. We aimed to identify the protein pathways disrupted as a consequence of chronic n-3 deficiency in the hippocampus of mice. Fatty acid analysis of the hippocampus following chronic dietary deficiency revealed a 3-fold decrease (p < 0.001) in n-3 FA levels. Label free LC-MS/MS analysis identified and profiled 1008 proteins, of which 114 were observed to be differentially expressed between n-3 deficient and control groups (n=8 per group). The cellular processes that were most implicated were neuritogenesis, endocytosis, and exocytosis, while specific protein pathways that were most significantly dysregulated were mitochondrial dysfunction and clathrin mediated endocytosis (CME). In order to characterise whether these processes and pathways are ones influenced by antipsychotic medication, we used LC-MS/MS to test the differential expression of these 114 proteins in the hippocampus of mice chronically treated with the antipsychotic agent haloperidol. We observed 23 of the 114 proteins to be differentially expressed, 17 of which were altered in the opposite direction to that observed following n-3 deficiency. Overall, our findings point to disturbed synaptic function, neuritogenesis, and mitochondrial function as a consequence of dietary deficiency in n-3 FA. This study greatly aids our understanding of the molecular mechanism by which n-3 deficiency impairs normal brain function, and provides clues as to how n-3 FA exert their therapeutic effect in early psychosis.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-10-23_05:01:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Caitriona Scaife
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-09-01 07:15:40	ID requested
1	2014-09-02 04:15:18	announced
2	2014-09-03 00:30:28	announced	Updated project metadata.
⏵ 3	2014-10-23 05:01:21	announced	Updated project metadata.

English JA, Harauma A, F, ö, cking M, Wynne K, Scaife C, Cagney G, Moriguchi T, Cotter DR, Omega-3 fatty acid deficiency disrupts endocytosis, neuritogenesis, and mitochondrial protein pathways in the mouse hippocampus. Front Genet, 4():208(2013) [pubmed]

curator keyword: Biological

submitter keyword: Omega-3, proteomics, neuritogenesis, mitochondrial dysfunction, clathrin mediated endocytosis, schizophrenia

David R. Cotter
contact affiliation	Department of Psychiatry, Royal College of Surgeons in Ireland, ERC Beaumont Hospital, Dublin, Ireland
contact email	drcotter@rcsi.ie
lab head
Caitriona Scaife
contact affiliation	University College Dublin
contact email	caitriona.scaife@ucd.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/09/PXD001277

PRIDE project URI

• PRIDE
  1. PXD001277
     1. Label: PRIDE project
     2. Name: Mouse, Hippocampus, LC-MSMS